研究了CuTAPc-Fe3O4复合粒子的磁性能、热稳定性、抗氧化性、溶解性和贮存稳定性,表明由于CuTAPc在Fe3O4纳米粒子表面形成了复合层,显著提高了CuTAPc-Fe3O4复合粒子的稳定性.将CuTAPc-Fe3O4复合粒子作为载体,在0℃以pH5.0的2mg/mL漆酶磷酸缓冲液为应用液进行漆酶固定,固载率为20%;以ABTS作为底物,固定化漆酶的最适pH为3.0,最适催化温度为45℃,Km值为23.8μmol/L.
The magnetic property, thermal stability, antioxidization abilitity, resolvable abilitity and storage stability were studied in detail. When CuTAPc formed covering layers on the surface of the composite, the stabilities of the composite are improved greatly. When pycnoporus sanguineus laccase was immobilized on CuTAPc-Fe3O4 nanoparticles composite in 2.0 mg/mL laccase phosphate buffer solution at pH 5.0 and 0℃, the immobilization yield was 20%. When ABTS was used as a substrate, the optimal pH, the optimal temperature and the Km value of the immobilized laccase were pH 3.0, 45℃ and 23.8 μmol/L respectively.