自东北三省蔬菜大棚内采集38株未嫁接黄瓜的枯萎病病株,采用组织分离法,共获得376株镰孢菌,根据形态鉴定,确定属于6种镰孢菌,其中尖孢镰孢(Fusarium oxysporum)占82.18%,木贼镰孢(F. equiseti)占9.31%,腐皮镰孢(F. solani)占4.52%,半裸镰孢(F.semitectum)占2.93%,串珠镰孢(F. monilifrorme)和锐顶镰孢(F. acuminatum)分别只占0.53%.从辽宁省采集的8株长势弱的嫁接黄瓜植株根茎上分离到20株镰孢菌,分别为串珠镰孢(8株)、尖孢镰孢(7株)、半裸镰孢(4株)和木贼镰孢(1株).
376 isolates of Fusarium species were obtained from 38 cucumber plants showing wilt symptom in vegetable greenhouses in Northeastern China. Based on morphological characteristics, these isolates were identified to 6 species, namely F. oxysporum(82.1%), F. equiseti(9.31%), F. solani(4.52%), F. semitectum(2.93%), F. moniliforme(0.53%) and F. acuminatum(0.53%). 20 Fusarium isolates were got from 8 grafted cucumber plants growing weakly in vegetable greenhouses in Liaoning Province, they belong to F. monilifotme(8 isolates), F, oxyspornm(7 isolates), F. semitectum(4 isolates) and F. equiseti(l isolate).