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Optimization of Two-species Whole-cell Immobilization System Constructed with Marine-derived Fungi and Its Biological Degradation Ability
  • ISSN号:1004-9541
  • 期刊名称:《中国化学工程学报:英文版》
  • 时间:0
  • 分类:X703[环境科学与工程—环境工程] TS71[轻工技术与工程—制浆造纸工程]
  • 作者机构:[1]Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
  • 相关基金:Supported by the National Natural Science Foundation of China (21036005), Scientific Technology Program of Zhejiang Province (2011C33016).
中文摘要:

在耕作的过程自发地形成的 Mycelia 小团由于它的唯一的结构的特征为整个房间的固定作为一个生物搬运人被利用。一个创新二种类的整个房间的固定系统被接种导出水兵的真菌 Pestalotiopsis sp 完成。进包含另一真菌毛丛 janthinellum P1 的文化媒介的 J63 孢子预先成长为没有任何预告的处理的 2 天的 mycelia 小团。为了评估这篇小说的生物降级能力,构造了固定系统,使不能调动的小团被使用对待造纸厂自河并且将染料脱色蔚蓝的 B。在自河的构造固定系统的使用导致了众多的不可溶解的好纤维的成功、快速的 biodegradation。为最大的 biodegradation 能力的使不能调动的过程的最佳条件与 P1 小团的生物资源用直角的设计被决定 10 g (湿团) , J63 孢子 2 的集中 ????  ??嘠 吗??

英文摘要:

Mycelia pellet formed spontaneously in the process of cultivation was exploited as a biological carrier for whole-cell immobilization due to its unique structural characteristic. An innovative two-species whole-cell im- mobilization system was achieved by inoculating the marine-derived fungus Pestalotiopsis sp. J63 spores into cul- ture medium containing another fungus Penicillium janthinellum P1 pre-grown mycelia pellets for 2 days without any pretreatment. In order to evaluate the biological degradation capacity of this novel constructed immobilization system, the immobilized pellets were applied to treat paper mill effluent and decolorize dye Azure B. The use of the constructed immobilization system in the effluent resulted in successful and rapid biodegradation of numerous in- soluble fine fibers. The optimum conditions of immobilized procedure for maximum biodegradation capacity were determined using orthogonal design with biomass of P1 pellets 10 g (wet mass), concentration of J63 spore 2x109 mlq, and immobilization time 2 d. The results demonstrate that immobilized pellets have more than 99% biodegradation capacity in a ten-hour treatment process. The kinetics of biodegradation fits the Michaelis-Menten equation well. Besides, the decolorization capability of immobilized pellets is more superior than that of P1 mycelia pellets. Overall, the present study offers a simple and reproducible way to construct a two-species whole-cell immobiliza- tion system for sewage treatment.

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期刊信息
  • 《中国化学工程学报:英文版》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国化学工业与化学工程学会
  • 主编:
  • 地址:北京东城区青年湖路13号
  • 邮编:100011
  • 邮箱:cjche@cip.com.cn
  • 电话:010-64519487/88
  • 国际标准刊号:ISSN:1004-9541
  • 国内统一刊号:ISSN:11-3270/TQ
  • 邮发代号:
  • 获奖情况:
  • 1998年化工系统优秀信息成果一等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
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  • 被引量:385