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Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications

ISSN号：1003-9015
期刊名称：《高校化学工程学报》
时间：0
分类：TQ658[化学工程—精细化工] TB39[一般工业技术—材料科学与工程]
作者机构：[1]State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China, [2]Department of Chemical and Biological Engineenng, Zhejiang University, Hangzhou 310027, China
相关基金：Acknowledgements This work was supported by the National Natural Science Foundation of China （Grant Nos. 20876145, 21036005）, the Science and Technology Cooperation Project between China-Europe Country＇s Governments from the Ministry of Science and Technology of China （No. 1017） and the Zhejiang Provincial Natural Science Foundation of China （No. Y4080326）.

作者： Dongjiao ZHOU[1], Shaochuan SHEN[1], Junxian YUN[1], Kejian YAO[1], Dong-Qiang LIN[2]

关键词：组织工程支架, 透明质酸, 葡聚糖, 制备, 共聚, 低温, 应用, 甲基丙烯酸甲酯, cryogel scaffold, tissue engineering, dextran,hyaluronate, 3T3-L1 preadipocyte

中文摘要：

Dextran-hyaluronate (Dex -- 哈) 为软织物工程的基于的 supermacroporous cryogel 脚手架被在结冰条件下面在各种各样的 macromonomer 集中包含葡聚糖 methacrylate (Dex 麻省) 和 hyaluronate methacrylate (哈麻省) 的水的答案的免费激进的 cryo-copolymerization 准备。这被观察为 Dex 的准备的 macromonomers 的合适的全部的集中 -- 哈有满足的性质的 cryogel 支架是 5%(w/w ) 在 1% 的哈麻省集中(w/v ) ，它然后被用来生产测试支架。有 5% 的获得的 cryogel 支架(w/w ) macromonomer 答案有高水位线渗透(5.1 ? 桴 ? 潨灳瑩？

英文摘要：

Dextran-hyaluronate （Dex-HA） based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate （Dex-MA） and hyaluronate methacrylate （HA-MA） at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concen- tration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% （w/w） at the HA-MA concentration of 1% （w/v）, which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% （w/w） macromonomer solution had high water permeability （5.1 × 10 ^-2m2） and high porosity （92.4%）. The pore diameter examined by scanning electron microscopy （SEM） was in a broad range of 50-135 um with the mean pore diameter of 91 um. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.