欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
磺酸基团是用来分离乳铁蛋白普遍被认可的配基，因此含有磺酸基团的层析剂是被用得最广泛的分离介质。通过对三种接有磺酸基团的层析介质，在不同pH和盐浓度时的静态吸附研究，得到了吸附的基本规律：随着pH或盐浓度的增大，吸附容量降低。利用分子模拟技术，比较了乳铁蛋白分子表面电势在不同条件时的变化，推测pH影响吸附的机理在于改变了蛋白表面氨基酸残基的质子化状态和分子表面电势，而盐浓度主要是屏蔽了蛋白和配基之间的相互作用，改变蛋白分子表面整体电势强弱，对电势分布没有明显影响。通过计算蛋白和配基之间的结合能，量化不同条件下的吸附能力，表征pH和盐浓度的影响。
英文摘要：
Sulfonate group is the most common ligand used for purifying lactoferrin. The isotherm adsorption curves of lactoferrin on three adsorbents with sulfonic ligand at different pHs and salt concentrations showed that adsorption capacity decreased with the increase of pH or salt concentration. Further molecular simulation was performed to compare the electrostatic potential of lactoferrin under different conditions. It could be presumed that the mechanism of pH effect was the change of protonation of amino acid residues on the surface of protein and the change of electrostatic potential consequently, while the mechanism of salt effect could be the shield of electrostatic interaction between protein and ligand with no impact on electrostatic potential distribution obviously. Finally, the binding energy between protein and ligand under different conditions was calculated to quantify adsorption capacity and characterize the effects of pH and salt concentration.
同期刊论文项目
同项目期刊论文
Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnologic
Preparation of ibuprofen-loaded chitosan films for oral mucosal drug delivery using supercritical so
Preparation of chitosan microparticles with diverse molecular weights using supercritical fluid assi
Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-e
Enhancement of Laccase Activity by Marine-derived Deuteromycete Pestalotiopsis sp J63 with Agricultu
New hydrophobic charge-induction resin with 2-mercaptoimidazole as the ligand and its separation cha
Immiscible liquid-liquid slug flow characteristics in the generation of aqueous drops within a recta
Novel NaCS-CS-PPS microcapsules as a potential enzyme-triggered release carrier for highly-loading 5
Protein adsorption behavior and immunoglobulin separation with a mixed-mode resin based on p-aminohi
Effect and mechanism of sodium chloride on the formation of chitosan-cellulose sulfate-tripolyphosph
Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expres
Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing fee
Effects of ligand density and pore size on the adsorption of bovine IgG with DEAE ion-exchange resin
Purification of Immunoglobulin of Egg Yolk with Hydrophobic Charge Induction Chromatography: Compari
Evaluation of a PEG/hydroxypropyl starch aqueous two-phase system for the separation of monoclonal a
Preparation and evaluation of dextran-grafted agarose resin for hydrophobic charge-induction chromat
Rapid freezing cryo-polymerization and microchannel liquid-flow focusing for cryogel beads: Adsorben
Adsorption of rutin with a novel beta-cyclodextrin polymer adsorbent: Thermodynamic and kinetic stud
Enhancing the activity and thermostability of thermostable beta-glucosidase from a marine Aspergillu
Formulation of Poorly Water-Soluble Compound Loaded Solid Lipid Nanoparticles in a Microchannel Syst
Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds fo
Preparation of Cellulose Adsorbents with Ionic Liquid and Pore Expansion for Chromatographic Applica
Molecular recognition of Fc-specific ligands binding onto the consensus binding site of IgG: insight
An integrated expanded bed adsorption process for lactoferrin and immunoglobulin G purification from
One-Step Purification of Lactoferrin from Crude Sweet Whey Using Cation-Exchange Expanded Bed Adsorp
Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads fo
Separation of Recombinant Geranylgeranyl Diphosphate Synthase of Deinococcus radiodurans from Expres
A Novel Method for the Preparation of Spherical Cellulose-Tungsten Carbide Composite Matrix with NMM
Preparation of micro-scaled multilayer capsules of poly-dimethyl-diallyl-ammonium chloride and sodiu
Evaluation of poly(ethylene glycol)/hydroxypropyl starch aqueous two-phase system for immunoglobulin
Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel be
Influences of Ligand Structure and pH on the Adsorption with Hydrophobic Charge Induction Adsorbents
Microbial Side-Chain Cleavage of Phytosterols by Mycobacteria in Vegetable Oil/Aqueous Two-Phase Sys
期刊信息
位置：