为了探究乳酸乳球菌KLDS4.0325的B族维生素合成潜力,利用各类B族维生素生物合成途径的相关蛋白序列针对该菌株的氨基酸序列进行同源性搜索,并与其他9株乳酸乳球菌的叶酸生物合成途径进行比较分析.结果表明:与参考菌株相比,乳酸乳球菌KLDS4.0325具有较为完整的叶酸和核黄素合成途径编码基因,在基因水平上可以有效合成叶酸和核黄素,具有相当大的工业潜能.
In order to exploit the potential of Lactococcus lactis subsp.lactis KLDS4.0325 for synthesizing B-group vitamins,the sequences of proteins involved in the biosynthetic pathways of all kinds of B-group vitamins were used to perform a BLASTp search against the amino acid sequence ofL.lactis KLDS4.0325,and comparative analysis with other 9 L.lactis strains.Results showed that compared with the reference strains,L.lactis KLDS4.0325 possessed relatively complete genes encoding proteins involved in the biosynthetic pathways of folate and riboflavin,could efficiently synthesize folate and riboflavin at the genetic level.Thus this strain will have a number of potential applications.