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中文摘要：
目的探讨大气沙尘与非沙尘天气细颗粒物（PM25）对人肺腺癌上皮细胞炎性因子分泌的影响。方法沙尘与非沙尘细颗粒物采自甘肃省武威市;实验细胞为人肺腺癌上皮A549细胞（A549细胞）。分别用25,50,100,200μg/ml沙尘与非沙尘细颗粒物对A549细胞进行染毒,染毒24 h后,细胞计数法测定细胞毒性。收集细胞上清,用放射免疫法测定细胞因子白介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的浓度。结果（1）沙尘细颗粒物对A549细胞作用24 h后,在细胞毒性上表现出低浓度促进细胞增殖,高浓度抑制增殖现象;非沙尘颗粒物随染毒剂量增加,细胞存活率明显降低,抑制细胞增殖。（2）沙尘、非沙尘细颗粒物对A549细胞作用24 h后,均可促进A549细胞分泌炎性因子IL-8。（3）沙尘、非沙尘颗粒物对A549细胞作用24 h后,对TNF-α分泌的影响不明显。结论沙尘与非沙尘细颗粒物均有早期炎症效应。
英文摘要：
Objective To study the effects of PM25 collected in duststorm and normal weather days on the secretion of interleukin-8（IL-8） and tumor necrosis factor-α（TNF-α） human lung adenocarcinoma A549 cells （A549 cells）. Methods PM2.5 was collected during duststorm and normal weather days in Wuwei, a city of Gansu province. A549 cells were exposed to PM2.5 at the doses of 25, 50,100,200 μg/ml, respectively. After 24 hours, cytotoxicity of the PM2.5 was measured by cell number counting. The levels of IL-8 and TNF-α were measured using radio-immunity assay. Results PM2.5 collected in duststorm increased the proliferation of A549 cells at low doses while inhibited it at high doses. PM2.5 collected in duststorm and normal weather days, however, inhibited at the tested concentrations. Both kind of PM2.5 increased the secretion of IL-8. However, there were no significant changes in the secretion of TNF-α from cells treated by both kind of PM2.5-Conclusion PM2.5 from the sandstorm dust and non-sandstorm dust could induce the early inflammatory reaction in the A549 cells.
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