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SO2对大鼠肺基因组表达谱的影响——环境表达不稳定基因组存在的证据

ISSN号：0253-2395
期刊名称：《山西大学学报：自然科学版》
时间：0
分类：Q355[生物学—遗传学] X503.2[环境科学与工程—环境工程]
作者机构：[1]山西大学环境医学与毒理学研究所,山西太原030006
相关基金：国家自然科学基金（30230310;20477023）;山西省自然科学基金（20031092）

作者：孟紫强[1], 秦国华[1], 白巨利[1], 张建彪[1], 杨振华[1], 张欣[1]

关键词：二氧化硫, 肺, 大鼠, 基因芯片, 基因表达, 环境表达不稳定基因组, sulfur dioxide , lung , rat, GeneChip analysis , gene expression , expression unsteady genome to en-viroment

中文摘要：

采用Affymetrix的基因芯片（RAE230A）研究了SO2短期（56mg／m^3，6h／day，for 7 days）及长期（14mg／m^3，1h／day，for 30 days）吸入后时大鼠肺基因表达谱改变的影响．结果表明，与其相应的时照组相比，SO2短期吸入后表达上调的基因有31个，其中包括18个已知基因和13个新基因，而表达下调的基因有30个，其中包括19个已知基因和11个新基因；SO2长期吸入后表达上调的基因有173个，其中包括79个已知基因和94个新基因，而表达下调的基因有85个，其中包括46个已知基因和39个新基因．由此结论：1）高剂量短期吸入与低剂量长期吸入SO：在体内的作用机理不同；2）短期吸入SO2的大鼠肺基因表达谱与其时照组相比，氧化磷酸化相关的一些基因发生了变化，这表明高剂量短期吸入SO2可能导致线粒体功能的恶化；3）长期吸入SO2的大鼠肺基因表达谱与其对照组相比，表达有差异的基因涉及到脂肪酸代谢、免疫、炎症、氧化应激、原癌基因、肿瘤抑制基因和细胞外基质等，表明低剂量长期吸入SO2在体内的机理要更为复杂．本研究证明，在基因组中存在一些基因，它们的表达时环境污染物很敏感，可被称为环境表达不稳定基因组．

英文摘要：

Affymetrix GeneChip （RAE230A） analysis was carried to analyze the gene expression profiles of the lungs of Wistar rats short-term （20ppm, 6 h/day, for 7 days） and long-term （5 ppm, 1 h/day, for 30 days） exposed to SO2. The results showed that 31 genes, containing 18 known genes and 13 novel genes, were up-regulated ,and 30 genes, containing 19 known genes and 11 novel genes, were down-regulated in rats short-term exposed to SO2 compared with the control rats. While there were 173 genes, containing 79 known genes and 94 novel genes, were up-regulated, and 85 genes, containing 46 known genes and 39 novel genes, were down-regulated in rats long-term exposed to SOz compared with control rats. These results lead to the conclusions：（1）SO2 exerted its effects by different mechanisms in vivo at high-dose shortterm inhalation and at low-dose long-term inhalation; （2） A notable feature of the gene expression profile was the changed expression of genes related to oxidative phosphorylation in lungs of rats short-term ex- posed to SOz, which shows high-dose short-term exposed to SO2 may cause the deterioration of mitochondrial functions; （3）Discriminating genes in lungs of rats long-term exposed to SO2 included those involved in fatty acid metabolism, immune, inflammatory, oxidative stress, oncogene, tumor suppresser and extracellular matrix. The mechanism of low-dose long-term exposed to SO2 is more complex. It was proved that there was an expression unsteady genome to SO2 or environmental pollutants in total genome of rat lung.

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