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中文摘要：
应用全细胞膜片钳技术，研究SO2体内衍生物和食品添加剂——焦亚硫酸钠（sodium metabisulfite，SMB）对大鼠背根节（DRG）神经元瞬间外向钾电流（TOCs）和延迟整流钾电流（IK）的影响．结果表明，SMB可增大TOCs和IK，具剂量依赖性和电压依赖性．10μmol L^-1的SMB不影响TOCs的激活过程，给药前后TOCs的半数激活电压为（3．15±2．29）mV和（4．72±1．92）mV（N=8，P〉0．05），不改变其斜率；但10μmolL^-1的SMB却非常显著地影响TOCs的失活过程，给药前后其半数失活电压分别为（-53．6±0．45）mV和（-38．85±0．68）mV（N=8，P〈0．01），也不改变其斜率．10μmolL^-1的SMB显著提前IK的激活过程，给药前后IK的半数激活电压为（-11．98±3．50）mV和（-33．21±5．67）mV（N=8，P〈0．01），不改变其斜率．SMB显著增大TOCs和气，使IK的激活过程提前，抑制TOCs的失活过程，从而导致细胞内K^＋通过K^＋通道外流增加，进而可能对DRG神经元功能产生不利影响．图6参16
英文摘要：
The effects of SO2 derivative - SMB, a food preservative, on transient outward potassium currents （TOCs） and delayed rectifier potassium currents （ IK ） in rat DRG neurons were investigated using whole cell configuration of patch-clamp technique. The results showed that SMB increased the amplitudes of TOCs and IK in a concentration-and voltage-dependent manner. 10 μmol L^-1 SMB did not affect the activation process of TOGs. Before and after application of 10 μmol L^-1 SMB, the half - activation voltages of TOCs were （3.15 ± 2.29） mV and （4.72 ± 1.92） mV （ N = 8, P 〉 0.05 ）, respectively, and their slope factors were not changed. But the inactivation curve of TOCs was shifted to positive potentials, the half - inactivation voltages of TOGs were （ - 53.6 ± 0.45） mV and （ - 38.85 ± 0.68） mV （N = 8, P 〈 0.01 ）, without changing the slope factors too. 10 μmol L^-1 SMB affected the activation process of IK. The half-activation voltages of IK were （ -11.98 ±3.50） mV and （ - 33.21 ± 5.67） mV （N = 8, P 〈 0.01 ）, and their slope factors were not changed before and after application of 10 μmol L^-1 SMB. The enhancement effects of SMB on TOGs and IK were mainly due to the shift of the steady-state activation curve of IK in the hyperpolarizing direction and the shift of steady - inactivation of TOGs to more positive potentials. It is suggested that SO2 derivative-SMB cause the increase of extraeellular K ^＋ and induce the disturbance of rat DRG neuronal functions. Fig 6, Ref 16
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