欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
模拟人体生理条件（pH=7.4）,用荧光光谱法结合多元曲线分辨-交替最小二乘法（MCR-ALS）研究盐酸克伦特罗（Clen）与牛血清白蛋白（BSA）的相互作用。实验采用同一滴加模式及荧光和紫外光谱方法研究盐酸克伦特罗与BSA的相互作用,对经典的荧光和紫外光谱数据矩阵加以扩展,增加实验数据的信息量;由渐进因子分析法（EFA）得到的因子数和体系中各组分的浓度初值后,应用MCR-ALS对该扩展的2种光谱矩阵进行迭代计算,较好地分辨出传统方法无法得到的动态作用中各种物质的光谱图和浓度变化趋势图,并根据浓度趋势图计算出Clen与BSA的表观结合常数和结合比,结果与用双对数方程得到的结合常数和作用位点数吻合。通过同步荧光光谱法进一步发现克伦特罗对BSA的构象有一定的影响。
英文摘要：
Under the imitated physiological conditions（pH=7.4）,the interaction between clenbuteral（Clen） and bovine serum albumin（BSA） was investigated by the fluorescence and UV-vis spectroscopy using the multivariate curve resolution-alternating least squares method（MCR-ALS）.In this work,two different kinds of spectral methods with the same titration mode carried out.The two spectral data matrices were augmented and EFA was utilized to this data matrix to gain the factors and the initial estimate of the concentration profiles,then MCR-ALS constrained optimization was applied.The concentration profiles and pure spectra for each species,the calculation of the ： molar ratio in the complex and the apparent equilibrium constant were extracted.The binding constants Ka and the number of binding sites n were also evaluated from the double-logarithm curve.The recovered information compared MCR-ALS with the conventional method showed that the application of the chemometrics approach could lead to satisfactory spectral data interpretation and to obtain the qualitative and quantitative information which was difficult obtained conventionally.
同期刊论文项目
同项目期刊论文
Synchronous fluorescence and UV-vis spectrometric study of the competitive interaction of chlorproma
Simultaneous spectrophotometric determination of maltol, ethyl maltol, vanillin and ethyl vanillin i
Electrochemical and spectral investigation on interaction between riboflavin with deoxyribonucleic a
Multicomponent determination of organophosphorus pesticides in grain samples by linear sweep strippi
Simultaneous kinetic-spectrophotometric determination of three organophosphorus pesticides with mult
Synchronous fluorescence, UV-visible spectrophotometric, and voltammetric studies of the competitive
Synchronous fluorimetric determination of the residues of three quinolones in eel by partial least s
Simultaneous synchronous fluorescence determination of carbaryl, propoxur, and carbofuran with multi
Small molecule-biopolymer interactions: Ultraviolet-visible and fluorescence spectroscopy and chemom
Behavior and quantification studies of phoxim pesticide using cyclic and linear sweep stripping volt
Authentication of vegetable oils on the basis of their physico-chemical properties with the aid of c
Differential kinetic spectrophotometric determination of chlorpromazine hydrochloride and promethazi
Simultaneous determination of three synthetic glucocorticoids by differential pulse stripping voltam
Fluorescence spectrometric study on the interaction between umbelliferone and bovine serum albumin w
Fluorescence spectrometric study on the interactions of isoprocarb and scidium 2-isopropylphenate wi
Spectrophotofluorimetric study of the interaction between Trazodone hydrochloride and bovine serum a
Application of multivariate calibration methods for the simultaneous multiwavelength spectrophotomet
Simultaneous enzymatic kinetic determination of pesticides, carbaryl and phoxim, with the aid of che
Simultaneous spectrophotometric determination of certain beta-lactam antibiotics in rabbit serum usi
Interaction between quercetin-copper(II) complex and DNA with the use of the Neutral Red dye fluorop
Application of high performance liquid chromatography for the profiling of complex chemical mixtures
Simultaneous determination of iron and aluminium by differential kinetic spectrophotometric method a
期刊信息
位置：