在应激敏感品种皮特兰猪和抗应激品种二花脸猪为亲本建立的F2资源群体中选取52头140-150日龄、体重为65±5kg的猪。2h运输应激试验后静脉注射巴比妥钠麻醉屠宰取样。在已知的应激性状QTL座位区域内选择了22个微卫星标记,对各微卫星标记与运输应激后的pH45min、pH24h、嫩度、系水力、蒸煮损失以及肉色评分(1^*,a^*,b^*)6项肉质指标进行了相关性分析。结果表明,各位点等位基因数为3-8个,杂合度为0.4155-0.7432,多态信息含量为0.3651-0.8150。方差分析显示,S0101对肉色a*有极显著影响(P〈0.01);SW1023对系水力有显著影响(P〈0.05);SW1808对系水力有极显著影响(P〈0.01),对肉色1^*和a^*有显著影响(P〈0.05);S0112和S0092对pH45min有显著影响(P〈0.05)。研究结果表明在应激QTL座位内选取的这5个微卫星标记对运输后的猪肉品质存在显著影响。
The effects of 22 microsatellite DNA loci on meat quality related to stress following transport trial in pigs were analyzed. These subjects were selected from the segregating F2 generation of a Pietrain (PIE)(stress sensibility)×Erhualian (EHL)(stress tolerance) cross, pH45~an, pH24h, tenderness, water holding capacity, cooking loss, and color scores- 1^*, a^*, b^* of the pork were measured after the transport of 2 hours. One-way ANOVA was applied to analyze the relation between microsatellite polymorphisms and meat quality. The results revealed that the allelic number was 3-8, heterozygosity was 0.42-0.74 and polymorphism information content was 0.37-0.82. S0101 had significant effect on meat color-a^* (P 〈0.01). The effect of SW1023 on water holding capacity (WHC) was significant (P 〈0.05). The effects of SW1808 on WHC (P 〈0.01), meat color-l*(P 〈0.05) and b^*(P 〈0.05) were significant, respectively. Both S0112 and S0092 had significant effects on pH45min (P 〈0.05). Result suggested that these 5 microsatellites in swine stress QTL regions were closely associated with meat quality in response to transport.