中文摘要：

【目的】探讨不同时间运输应激对猪脾脏中IL-2mRNA、IL-6mRNA、IL-10mRNA及其IL-2RmRNA、IL-6RmRNA表达的影响，并探讨P38MAPK—NF—κB信号途径对其表达的调控作用。【方法】将19头体重50kg左右的猪随机分为4组，分别进行0、1、2和4h公路运输，运输结束后立即扑杀取脾脏，液氮保存，用荧光定量PCR的方法对3种细胞因子及其IL-2RmRNA、IL-6RmRNA进行定量；另用ELISA方法检测试验猪脾脏组织匀浆液中P38MAPK含量的动态变化；激光扫描共聚焦显微镜观察NF-κB运输前后在脾脏细胞中的分布。【结果】经1、2和4h的运输应激后，脾脏中IL-10、IL-6及IL-6RmRNA的表达发生了显著的变化，P38MAPK变化不明显，NF—κB在脾脏中的表达是先上升，随后稍微下降，到4h时又升高并高于其它3个组；且NF—κB的入核率也呈现同样的变化趋势。【结论】运输应激可引起猪脾脏IL-6mRNA、IL-10mRNA和IL-6RmRNA表达的显著变化，且存在时效性；相关性分析表明，在所检测的3种细胞因子中，脾脏主要对运输过程中IL-6水平起重要的调节作用，结合细胞因子表达调控的信号机制表明，NF—κB可能对运输过程中猪脾脏相关细胞因子变化起重要的调控作用。

英文摘要：

[Objective] The objective of this study was to explore expressions of IL-2, IL-6, IL-10 mRNAs, IL-2RmRNA and IL-6RmRNA in spleen and regulation of cytokines by P38MAPK - NF-κB in pigs during truck transportation. [ Method] Nineteen pigs were randomly divided into 4 groups for 0 h, 1 h, 2 h and 4 h transportaion when mean live weight was approximately （50±2） kg （mean ± standard deviation; SD）. At the end of the transportation trial, the spleen was removed and frozen in liquid nitrogen. SYBR Green I-based one-step real-time quantitative RT-PCR amplification was performed in quantitation of the three cytokines, IL-2RmRNA and IL-6RmRNA. P38MAPK in the homogenization sample of spleen was measured with homologous specific enzyme-linked immunosorbent assay （ELISA）. The distribution of NF-Id3 in the spleen was examined using confocal laser scanning microscope （CLSM）. [Result] After 1 h, 2 h and 4 h of transportation, the expression level of IL-6, IL-10 and IL-6RmRNAs in spleen were significantly changed compared with the control group during transportation stress. NF-κB in the spleen has a sharp rise at 1 h, followed by a slight decline, rise again at 4 h and higher than the other three groups. The rates of NF-r,B entry nuclear showed a similar trend, but there was no significant change with respect to P38MAPK. [ Conclusion ] The IL-6mRNA, IL-10mRNA and the expression of IL-6RmRNA could be significantly changed by transportation stress and were also related to the time of post-stress in pigs. Correlation analysis showed that the spleen is an important regulator of IL-6 levels among the detected three kinds of cytokines during transportation stress. Combination of expression and regulation of cytokine signaling mechanism shows that the activation of NF-κB in the spleen may be play an important role in the peripheral immune-related cytokines change in transport-stressed pigs.