中文摘要：

仔猪运输能引起应激反应,从而增加对疾病的易感性,这与应激导致的免疫和抗氧化能力改变有关。本研究的目的是了解2 h运输对仔猪重要器官抗氧化功能和主要促炎细胞因子的影响,为进一步制定抗应激措施提供理论依据。将30头体质量约20 kg的仔猪平均分为试验组和对照组,试验组仔猪用卡车在公路上运输2 h,比较试验组和对照组仔猪肝脏和心脏抗氧化指标和血清IL-1β和IL-2的变化。结果显示,2 h运输对仔猪心脏抗氧化能力有一定的影响,试验组仔猪心脏活性氧（ROS）量[（14 536.0±4 313.5）AU.mg^-1]多于对照组仔猪[（12 778.0±2 589.6）AU.mg^-1],但无统计学差异（P〉0.05）;运输后仔猪肝脏总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性显著下降（P〈0.05）,脂质过氧化产物丙二醛（MDA）含量显著上升（P〈0.05）;肝脏和心脏中主要抗氧化酶——铜锌超氧化物歧化酶（Cu-Zn SOD）mRNA水平虽均有所降低,但与对照组相比差异不显著（P〉0.05）;运输后仔猪主要促炎细胞因子IL-1β和IL-2在血清中的含量与对照组相比虽无显著性差异（P〉0.05）,但均有所升高。以上结果表明,2 h运输可从基因转录和表达水平上降低仔猪肝脏的抗氧化功能,使肝细胞脂质过氧化程度增强,血清中促炎细胞因子的升高也可直接损伤组织,因此,抗氧化能力的下降和促炎细胞因子的生成增加,是运输仔猪组织损伤的重要原因。

英文摘要：

The transportation of piglets results in stress that is associated with increased susceptibility to diseases, presumably due to alteration of immune function and antioxidative ability. The objective of present study was to determine if a 2 h transportation of piglets by road induced changes in antioxidative ability and proinflammatory cytokines. Thirty piglets weighting about 20 kg were divided into experimental and control group on the average. The animals in experimental group were transported by truck on road for 2 h. Then hearts and livers were collected, blood was harvested from all piglets. The antioxidative capacity of hearts and livers and serum concentration of IL-1β and IL-2 were assayed. The result were as follows, the antioxidative ability was not obviously changed in hearts after transportation, although production of reactive oxygen species （ROS） in hearts of transported animals was greater than that of control group（P〉0.05）. In livers, the activities of total antioxidative capacity （T-AOC） and eatalase （CAT） were obviously decreased （P〈0. 05） following transportation, and the decrease was accompanied with significant rise of the maleic dialdehyde （MDA） concentration （P〈0.05）. Cu-Zn superoxide dismutase （Cu- Zn SOD）, a important antioxidative enzyme, was down-regulated in hearts and livers following transportation in mRNA level （P〉0.05）. The serum contents of proinflammatory cytokines IL- 1β and IL-2 were risen slightly. It was concluded that 2 h transportation of piglets induces decrease of antioxidative capacity only in the liver and increase of proinflammatory cytokines of IL- 1βand IL-2 concentration in sera slightly. It was indicated that the decreased antioxidative capacity and increased proinflammatory cytokine concentration lead to tissue lesion of piglets following transportation.