中文摘要：

运输引起的应激可增加仔猪对疾病的易感性，这与免疫功能的改变有关，本研究探讨2 h运输对二花脸仔猪细胞免疫机能的影响。将21头20 kg左右二花脸仔猪分成运输组和对照组，运输组7头，对照组14头，运输组仔猪在公路上运输2 h,运输结束后，所有仔猪用巴比妥钠麻醉后宰杀，取血液和脾脏。用放射性免疫法测定血清IL-1β和IL-2含量；用MTT法测定应激猪血清对正常猪外周血T淋巴细胞增殖的影响；用RT-PCR方法测定脾脏中IL-1β和IL-2 mRNA表达。结果：（1）运输猪血清中IL-1β和IL-2含量均升高，但与对照组相比无统计学差异（P＞0.05）；（2）1∶4和1∶8稀释的运输猪血清，能显著提高正常猪外周血T淋巴细胞增殖活性（P〈0.05）；（3）与对照猪相比， 运输猪脾脏中IL-2 mRNA表达量显著增高（P〈0.05），IL-1β mRNA表达变化不明显。本研究表明：2 h运输可改变仔猪的免疫状态，使机体代偿性地调动应急机制来应对不良环境。

英文摘要：

Transportation of piglets may result in stress that is associated with increased susceptibility of diseases, presumably due to alteration of immune function. The objective of this investigation was to explore effect of road transportation of Erhualian piglets on cell-mediated immune function. 21 Erhualian piglets about 20 kg in weight were divided into transportation and control groups, 7 in transportation group and 14 in control group. After 2 h transportation, all piglets were killed after anaesthesia with Pentobarbital Sodium. Blood and spleen samples were collected. IL-1β and IL-2 levels in serum were measured by radioimmunoassay. Effects of stressed piglet＇s serum on T-lymphocyte proliferation in peripheral blood lymphocytes （PBLs） from normal pigs were detected by methylthiazol tetrazolium bromide （MTT） assay. And expressions of IL-1β and IL-2 mRNA were determined by RT-PCR. The results were as follows： （1） IL-1β and IL-2 levels in serum from transportation piglets were higher than that of control group（P〉0.05）. （2） The serum of transportation stress piglets can obviously promote the activity of T-lymphocyte proliferation in PBLs from normal pigs（P〈0.05） at 1 ： 4 and 1 ： 8 dilution; （3） Expression of IL-2 gene in spleens were profoundly up-regulated （P〈0.05） by transportation stress, while expressions of IL-1β gene unchanged. The above results demonstrated that a 2 h transportation may alter immune function of piglets, and the piglets can adapt to this transportation manner well by strengthening their immune system function to cope with the harmful environment.