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中文摘要：
目的分析在人胚肺成纤维细胞（HELF）中热休克蛋白47（HSP47）与转化生长因子-β1（TGF-β1）之间的关系。方法将HELF分为4组：对照组、热休克组、TGF组及联合组。采用WST-8法检测HELF细胞活力和Western blot检测各组细胞HSP47、Ⅰ型胶原（ColⅠ）、α-平滑肌肌动蛋白（α-SMA）、波形蛋白（Vi mentin）表达情况。结果 TGF-β1能促进HELF的增殖（P〈0.05）;在TGF-β1作用下HELF中上述蛋白表达上调,在TGF-β1联合热休克反应的作用下上调更明显,而单在热休克反应后细胞HSP47表达显著,ColⅠ却未见明显增强（P〉0.05）。结论 HSP47参与TGF-β1促HELF胶原合成,在肺纤维化中起着重要的作用。
英文摘要：
Objective To analyze the relationship between heat shock protein 47（HSP47） and transforming growth factor beta 1（TGF-β1） in human embryonic lung fibroblasts（HELFS）.Methods HELFS were divided into four groups：the control group,the heat shock group,TGF group and the combination group.HELF cell viability was measured by WST assay,the expressions of HSP47,Collagen-Ⅰ,α-smooth muscle actin（α-SMA） and Vimentin in cells were detected by Western blot.Results The cell viability was gradually increased after HELF being treated by TGF-β1（P0.05）;All proteins showed obvious expression in HELF induced by TGF-β1,increased more obviously induced by TGF-β1 combined with HSR.HSP47 was obviously expressed while Collagen-I was slightly expressed after HSR（P0.05）.Conclusion HSP47 may play an important role in pulmonary fibrosis as a downstream factor regulated by TGF-β1.
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