为给核果类果树组织培养的深入研究提供参考,综述了有关胚培养、茎尖培养、茎段培养、叶片培养及原生质体培养的研究进展,重点分析了基本培养基、碳源、激素种类与配比和环境条件等影响因子对培养效果的影响情况,指出了核果类果树组织培养中存在的问题,并且提出了解决这些问题的途径。
In order to provide reference for the tissue culture in the stone fruit trees of Rosaceae,the progress of tissue culture research was discussed,including culture of shoot tip,stem segment with bud,embryo culture protoplast and leaf.The effects of several major factors in procedure of tissue culture were reviewed,including basic media,carbon sources,hormone types and ratio and the other environmental conditions.Moreover,the existing questions in tissue culture of stone fruit trees were pointed out and corresponding solutions were also put forward.