中文摘要：

采用硅烷试剂对气相二氧化硅表面进行活化,并以此为载体固定N-乙酰基-L-半胱氨酸修饰的金纳米（NAC-金纳米）和酪氨酸酶,最后得到的复合物作为荧光探针,建立测定邻苯二酚的固体荧光猝灭分析方法。考察了各种实验条件对酪氨酸酶和NAC-金纳米固定到气相二氧化硅表面的影响,并对其进行优化。用荧光光谱仪对NAC-金纳米-酪氨酸酶-气相二氧化硅的固体荧光进行研究并加以应用。实验结果表明,随着通入不同浓度的邻苯二酚溶液,固体粉末的荧光被有效猝灭,基于此建立了测定邻苯二酚的固体荧光法。该方法的检出限为1.0×10^-7mol/L,线性范围为5.0×10^-7～4.0×10^-4mol/L。

英文摘要：

The N-aceltyl-L-cysteine（NAC）-Au NPs and tyrosinase were immobilized on fumed silica which was previously activated by silane agents.The NAC-Au NPs-tyrosinase-fumed silica composite was used as fluorescence probes for the quantitative determination of catechol by solid fluorescence quenching method.The experimental parameters that impacted the immobilization of NAC-Au NPs and tyrosinase on fumed silica have been studied in the determination of catechol.The solid fluorescence of NAC-Au NPs-tyrosinase-fumed silica was studied and applied.The results showed that the solid fluorescence intensity of composite decreased upon increasing concentration of catechol.A linear range 5.0×10^-7 to 4.0×10^-4 mol/L and a detection limit 1.0×10^-7 mol/L of catechol were obtained.