对黑豆皮花青素不同提取方法,传统有机溶剂法、水浸提法、超声波辅助法的提取率进行了比较,结果表明:超声波辅助法的提取率最高,达到20.50%。并采用滤纸片法对3种不同提取方法所得的花青素抑菌效果进行了比较,结果显示:水浸提法对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌3种细菌的抑菌圈均最大,抑菌效果最好。另采用平板菌落计数法对水浸提法所得的花青素抑菌率进行比较发现,从低浓度到高浓度抑菌效果逐渐增强。当花青素在浓度为0.3 g·m L^-1时,对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌3种细菌的抑菌率最高,分别达到79.75%、74.50%和81.60%。
The extraction yield of different extraction methods of anthocyanin in black soybean skin inculding the traditional method of organic solvent,water extraction,ultrasonic auxiliary method were compared,method of ultrasonic assisted extraction yield was the highest,reached 20. 50%. And by the method of filter,three different extraction methods of the bacteriostatic effect of anthocyanins were compared. The result showed that the bacteriostatic ring of the water extraction of Bacillus subtilis,Staphylococcus aureus,Escherichia coli was the largest and the bacteriostasis effect was the best. The anthocyanin bacteriostatic rate compared by the plate colony counting method of water extraction showed that from low concentration to high concentration bacteriostatic effect was increased. When anthocyanins in concentration was 0. 3 g·m L^- 1,Bacillus subtilis,Staphylococcus aureus,Escherichia coli antibacterial rate was 79. 75%,74. 50% and 81. 60%,respectively.