中文摘要：

目前对于植物体中丙酮酸脱氢酶激酶（pymvate dehydrogenase kinase，PDK）的研究，除了解其参与调节线粒体丙酮酸脱氢酶复合体的活性外，对其他调控机制尚不清楚。本实验室的前期研究结果表明，小麦丙酮酸脱氢酶激酶（TaPDK）在小麦（Triticum aestivum）遗传型不育系和相应的生理型不育系中的表达是不一致的。为进一步研究SQ．1诱导的小麦花药败育过程中调控TaPDK的作用因子，本研究采用酵母双杂交方法筛选小麦TaPDK的互作蛋白。将含有pGBKT7-PDK质粒的酵母（Saccharomy cescerevi—ae）菌株Y2HGold与文库酵母菌株Y187共同融合培养，在SD／-Ade／-His／．Leu／．Trp平板上划线培养，挑选直径大于2mm的克隆，在SD／-Ade／-His／．Leu／-Trp／AbA／X-α-Gal平板上划线，筛选蓝色克隆。其中，筛选得到一个新的结合蛋白，该蛋白被命名为增殖细胞核抗原（proliferating cell nuclear antigen，TaPCNA）。将花粉细胞的核复制分裂与TaPCNA的定量表达结合分析发现，TaPCNA在不育系与可育系的叶片中几乎不表达，但在花粉发育的各个时期中呈现高表达，尤其在生理型不育系花药中的表达量显著高于遗传型不育系和可育系，这表明在生理型不育系中，TaPCNA与小孢子的细胞周期发育更加密切相关。这为进一步研究生理型不育小孢子异常发育的细胞周期提供了基础资料。

英文摘要：

It has been documented that pyruvate dehydrogenase kinase（PDK） is involved in the regulation of pyruvate dehydrogenase complex activity in mitochondria. However, very little is known about the regulatory mechanism of PDK. Our previous study showed that there was difference in the expression of TaPDK between genetic sterile line and physiological sterile line. To further study the acting factor regulating TaPDK during anther abortion induced by SQ- 1, the interaction protein of TaPDK was screened via yeast two-hybrid technique. The yeast （Saccharomyces cerevisiae） strain Y2HGold containing pGBKT7-PDK plasmid and, library yeast strain Y187 were cultured by yeast mating. Then, the mated culture was incubated on SD/-Ade/- His/-Leu/-Trp plate before selection of clones with diameter larger than 2 mm, and further incubated on SD/- Ade/-His/-Leu/-Trp/AbA/X-a-Gal plate for screening blue clones. A new binding protein proliferating cell nuclear antigen was obtained, and was designated as proliferating cell nuclear antigen（TaPCNA）. The results from pollen development and quantitative expression indicated that the expression of TaPCNA was scarcely expressed in leaf, and mainly expressed in different stage of pollen development. Especially, its transcripts accumulated predominantly in physiological sterile line at bicellular and tricellular stage, which suggested that TaPCNA was closely involved with the cell cycle of microspore development, and provided the basic date for further study of the abnormal cell cycle of microspore development in physiological sterile line.