中文摘要：

为获得绵羊肺炎支原体贵州株P113基因生物信息学特征,应用DNAStar、Mega 5.0、Protparam、Protscale、IEBD等工具对其（GZ-QX1株）P113蛋白特性、结构和功能进行预测分析。结果显示,绵羊肺炎支原体GZ-QX1株P113基因序列大小为3 240bp,编码1 079个氨基酸,与绵羊肺炎支原体Y98标准株、四川SC01株、猪肺炎支原体P97、丝状支原体山羊亚种、山羊支原体山羊亚种的核苷酸序列同源性分别为99.9%、81.9%、60.4%、3.9%和5.2%。P113蛋白是分子质量约119ku的碱性蛋白,具有较多优势抗原表位;蛋白结构分析显示,P113蛋白无跨膜结构,有9个N糖基化位点,59个丝氨酸、16个苏氨酸的磷酸化位点,14种保守的特异性蛋白质激酶的结合位点;蛋白功能分析认为,P113可能是某信号传导通路的信号分子,也是一种具有良好抗原性的结构蛋白。

英文摘要：

The assay was aimed to access the biological characteristics of P113 protein in Mycoplasma ovipneumoniae（Mo）Guizhou strain（GZ-QX1）.The protein structure,characteristics and function of GZ-QX P113 protein were predicted and analyzed by DNASTAR,MEGA 5.0softwares,Protparam,Protscale and IEBD online tools in this study.The results showed that Mo GZ-QX1 P113 gene predicted is 3 240 bp,encoding 1 079 amino acid（AA）,and sharing nucleotides sequence identity 99.9% with Mo Y98 standard strains 81.9% with SC01 Sichuan strain,60.4% with Mycoplasma hyopneumoniae P97,3.9% with Mycoplasma mycoides and 5.2% with Mycoplasmacapripneumoniae.The molecular weight of P113 protein is 119 ku,with more advantages antigen epitope protein structure.Protein structure analysis showed that the protein had no transmembrane domain.There were 9N-glycosylation sites,59 serine,16threonine may be phosphorylated predicted and 14 conserved and specific phosphkinase binding sites.Protein functional analysis showed that P113 may function as a mediator in signaling pathway,but also a kind of good antigenic structure of proteins.