中文摘要：

目的探讨中间型单核细胞在子痫前期发病过程中的作用。方法选择2014年10月至2015年10月在江苏省盐城市滨海县人民医院诊断为子痫前期的孕妇52例，同期健康孕妇42例和健康未孕女性42例作为对照组。采用流式细胞技术检测外周血中间型单核细胞比例，及其细胞表面Toll样受体（Toll-like receptor，TLR）2、TLR4、CD64和髓系细胞触发受体-1（triggering receptor expressed on myeloid cell-1，TREM-1）的表达（以阳性细胞比例和平均荧光强度表示），以及中间型单核细胞内肿瘤坏死因子-α（tumor necrosis factor—α，TNF—α）和白细胞介素（interleukin，IL）-6含量（以平均荧光强度表示）。液相芯片技术检测各组血清IL-6、IL-1β、TNF—α、IL-8和IL-12P70浓度。采用两独立样本t检验、单因素方差分析、Mann—Whitney U检验、Kruskal Wallis检验和Pearson相关分析进行统计学处理。结果子痫前期组中间型单核细胞比例为10．4％（5．3％～19．9％），高于健康孕妇组[6．6％（4．9％～7．8％）1，2组均高于健康未孕组[3．8％（2．4％～5．0％）]（P值均〈0．01）。子痫前期组TLR4[（60．1±12．5）％与（24．9±8．8）％]和CD64阳性中间型单核细胞比例[（85．3±5．4）％与（67．4±7．5）％]均高于健康孕妇组，中间型单核细胞表面TLR4（50．3±10．2与26．8±8．6）、TREM-1（35．6±4．1与28．6±4．7）和CD64的平均荧光强度（39．8±5．2与28．9±4．8）也高于健康孕妇组（t值分别为15．416、13．437、11．898、7．707和10．454，P值均〈0．01）。子痫前期组中间型单核细胞内IL-6和TNF—α的平均荧光强度分别为32．3±4．7和44．6±6．3，均高于健康孕妇组（分别为28．6±3．5和36．7±8．3）（t值分别为4．239和5．245，P值均〈0．01）。子痫前期组血清IL-6、IL8和TNF—α浓度高于健康孕妇组和健康未孕组，差异均有统计学意义（P

英文摘要：

Objective To explore the role of peripheral blood intermediate monocytes in the pathogenesis ofpreeclampsia. Methods Fifty-two patients with established preeclampsia in Binhai County People＇s Hospital from October 2014 to October 2015, 42 healthy pregnant women and 42 healthy non-pregnant women were enrolled in this study. The percentage of intermediate monocyte subsets, ratio of positive cells and mean fluorescence intensity （MFI） of Toll-like receptor （TLR） 2, TLR4, CD64, and triggering receptor expressed on myeloid cell-1（TREM-1）, and MFI of intracellular tumor necrosis factor- α （TNF-α ） and interleukin （IL）-6 were evaluated by flow cytometry. The concentrations of IL-6, IL-8, IL-1β, IL-12P70 and TNF-α in serum were analyzed using Luminex liquid phase chip technology. Independent two samples Hest, analysis of variance, Mann-Whitney U test, Kruskal-Wallis test and Pearson correlation analysis were used for statistical analysis. Results Percentage of intermediate monocytes was higher in preeclampsia patients [10.4%（5.3± 19.9%）] than in healthy pregnant women [6.6%（4.9±7.8%）], and both were higher than in non-pregnant women [3.8%（2.4±5.0）%] （all P〈0.05）. The ratio of TLR4 and CD64 positive intermediate monocytes [（60.1 ± 12.5）% vs （24.9 ± 8.8）%; （85.3 ± 5.4）% vs （67.4 ±7.5）%] （t were 15.416 and 13.437, both P〈0.05）, and MFI of TLR4 （50.3± 10.2 vs 26.8± 8.6）, TREM-1（35.6±4.1 vs 28.6±4.7） and CD64 （39.8±5.2 vs 28.9±4.8） （t were 11.898, 7.707 and 10.454, all P〈0.05） were higher in preeclampsia patients than in healthy pregnant women. MFI of intracellular IL-6 （32.3 ± 4.7 vs 28.6± 3.5） and TNF- α （44.6 ± 6.3 vs 36.7 ± 8.3） in intermediate monocytes of preeclampsia patients was also significantly higher than that of healthy pregnant women （t were 4.239 and 5.245, both P〈0.05）. Serum concentrations of IL-6, IL-8 and TNF- α were higher in preeclamptic patients than in healthy pregnant women and non-pregn