中文摘要：

鲍曼不动杆菌作为一种医院内感染的病原菌,因其易于引起各类感染且耐药性强而受到广泛关注。快速改造鲍曼不动杆菌基因组的工具可有效促进其耐药机制的研究。本文就近些年来适用于鲍曼不动杆菌的遗传操作方法进行了总结,包括各种外源基因转入方法（电转化、自然转化、接合转移）和基因改造技术（等位基因交换、DNA重组工程、转座突变）,并对鲍曼不动杆菌的基因组编辑方法的改进作了初步展望。

英文摘要：

Acinetobacter baumannii is a nosocomial pathogen, which garners widespread attention for its infections and drug resistance. Tools to rapidly dissect the A. baumannii genome will facilitate the study of its pathogenic mechanism. Here, we summarized genetic manipulation tools for A. baumannii genome, including various methods for transformation of exogenous DNA into A. baumannii （electroporation, natural transformation, conjugation） and genome modification techniques （allelic exchange, recombineering, transposon mutagenesis）, and carried out a preliminary prospect on progress of genome editing tools for A. baumannii.