中文摘要：

[目的]探讨乌苏里藜芦生物碱单体计米丁（germidine）抗动脉血栓形成作用的机制。[方法]大鼠70只,随机分为7组：生理盐水（NS）组、计米丁5个剂量组和阳性对照药物赖氨匹林（LAS）组。测定各组大鼠给药后血流阻塞时间（OT）,以NS组为基准,计算不同给药组OT延长百分率。采用Born法评价计米丁的体内、外血小板聚集抑制活性。大鼠60只,随机分为6组：NS组,计米丁4个剂量组,以及阳性对照药物LAS组。大鼠给药后取血,制备富血小板血浆（PRP）和贫血小板血浆（PPP）。测定ADP诱导的血小板5 min最大聚集率。以NS组为基准,计算不同给药组血小板聚集抑制百分率。另取大鼠60只,随机分为6组：NS组,计米丁4个剂量组,和阳性对照药物赖氨匹林（LAS）组。取血,制备PRP和PPP,于PRP中分别加入不同浓度受试药物或NS。测定ADP诱导的血小板5min最大聚集率。以NS组为基准,计算不同给药组血小板聚集抑制百分率,并计算计米丁体外抑制血小板聚集的半数抑制浓度IC50。[结果]与NS组相比,大鼠静脉注射计米丁（1.3-20.0μg.kg^-1）后呈剂量依赖性OT延长,计米丁呈剂量依赖性地抑制大鼠体内（2.5-20.0μg.kg^-1）及体外（6.3-50.0μg.L-1）血小板最大聚集率,其体外抑制血小板聚集的半数抑制浓度IC50为29.3μg.L-1。[结论]计米丁具有抗动脉血栓形成作用,该作用与其抗血小板作用有关。

英文摘要：

[Objective] To study the effect of germidine against arterial thrombosis in rats as well as the possible mechanism.[Methods] Seventy rats were divided into 7 groups randomly： NS group,5 groups with different doses and positive control group（LAS）.Occlusion time（OT） was measured after dosing.Then,the OT increasing percentage of germidine-treated groups,according to OT of saline control group,was served as an index to evaluate anti-arterial thrombosis effects of the drug.Born’s turbidimetric method was used to examine the platelet aggregation inhibitory activity of germidine in vitro and in vivo.Then,60 rats were divided into 6 groups randomly： NS group,4 groups with different doses and LAS group. The blood was drawn to prepare platelet rich plasma （PRP） and platelet poor plasma （PPP）. ADP - induced 5 min maxi- mum platelet aggregation rates were tested after dosing. Then, the decreasing percentage of platelet aggregation rate was cal- culated according to that of NS group. Other 60 rats were divided into 6 groups randomly ： NS group ,4 groups with different doses and LAS group. PRP and PPP were obtained, different concentration of test drugs or saline were added into PRP, then ADP - induced 5 min maximum platelet aggregation rate were tested, finally the decreasing percentage of platelet aggregation rate was calculated compared with NS group, as well as IC50 was calculated. [ Results] In comparison with rats in NS group, the rats administered in 5 different doses （ 1.3 - 20.0 μg.kg^-1） of germidine showed significantly and dose - dependently prolonged occlusion time （OT） of carotid artery injured by electrical stimulation, and platelet aggregation was found to be inhibited by germidine dose - dependently,in both in vitro and in vivo. [ Conclusion ] Germidine has anti - arterial thrombosis effect,which is related to its potent platelet aggregation inhibitory activity.