中文摘要：

RNA干扰（RNAi）为基因治疗提供了一个全新的思路。但单一的RNAi治疗只针对单个基因,因而最终无法治愈多基因变异引起的肿瘤。为了克服RNAi单基因治疗的不足,研究人员提出了RNA组合干扰（combinatorial RNA interference,coRNAi）的治疗策略。coRNAi通过联合应用针对单个或多个靶基因的RNAi诱发物以及其他RNA或蛋白沉默因子实现沉默靶基因、抑制肿瘤生长和促进肿瘤细胞凋亡。本文重点介绍了coRNAi的主要联合方式以及在肿瘤基因治疗中的研究进展。

英文摘要：

RNA interference（RNAi） has been proved as a novel approach for gene therapy.However,RNAi mono-therapy only aims at single gene,it therefore may ultimately fail to cure cancers caused by polygene variation.To overcome the deficiency of RNAi mono-therapy,＂combinatorial RNA interference＂（coRNAi） was put forward as a new strategy.By co-expressing the inducers of RNAi triggering single or multiple targets directly and other RNA-or protein-based silencers,coRNAi keeps target genes silent,prevents carcinogenic progression and induces apoptosis of tumor cells.This paper mainly reviews the major strategies of coRNAi and their applications in cancer gene therapy.