中文摘要：

目的 探讨以屋尘螨（Dermatophagoides pteronyssinus）1类变应原T细胞表位融合肽（TAT-IhC-DPTCE）为疫苗，评价其对过敏性哮喘小鼠特异性免疫治疗的效果。 方法 120只SPF级BALB/c小鼠随机均分为PBS组（阴性对照，A组）、ProDer p 1变应原致敏组（B组）、ProDer p 1变应原免疫治疗组（C组）、DPTCE蛋白免疫治疗组（D组）、TAT-DPTCE蛋白免疫治疗组（E组）和TAT-IhC-DPTCE蛋白免疫治疗组（F组），每组20只。分别于第0、7、14天，A组小鼠腹腔注射PBS，B~F组小鼠均腹腔注射屋尘螨变应原提取液10 μg。第21天起，A组小鼠雾化吸入PBS，B～F组小鼠均吸入0.5 μg/ml屋尘螨变应原提取液，1次/d×30 min，连续7 d。C～F组小鼠于第25～27天雾化前30 min分别腹腔注射100 μg/ml ProDer p 1、DPTCE、TAT-DPTCE和TAT-IhC-DPTCE溶液各200 μl，进行特异性免疫治疗，A、B组小鼠分别注射200 μl PBS。最后1次雾化后24 h，处死各组小鼠。HE染色观察小鼠肺组织病理变化。分别收集各组20只小鼠支气管肺泡灌洗液（BALF），ELISA检测BALF中γ干扰素（IFN-γ）、白细胞介素13（IL-13）、IL-10和β转化生长因子（TGF-β）的水平，并计数嗜酸粒细胞数量（EOS）。分别取各组5只小鼠眼眶血，ELISA检测血清中变应原特异性IgE、IgG1和IgG2a的抗体水平。结果 HE染色镜检结果显示，与B组比较，F组小鼠支气管周围嗜酸粒细胞增多、上皮细胞脱落和支气管上皮细胞肥大等肺部炎症明显减轻。小鼠BALF中，F组的IFN-γ水平为（298.75±26.09）pg/ml，高于B组（158.71±20.89）pg/ml、C组（210.38±18.92）pg/ml、D组（229.44±13.00）pg/ml和E组（233.24±20.39）pg/ml（P〈0.01）；IL-10和TGF-β水平与IFN-γ相似，F组IL-10和TGF-β水平分别为（105.32±7.24）和（119±9.33）pg/ml，均高于B组（23.29±3.18）和（41.19±4.63）pg/ml、C组（43.54±4.28）和（60.19±6.47）pg/ml、D组（51.33±6.19）和（69.34±

英文摘要：

Objective To study the specific immunotherapeutic effect of Dermatophagoides pteronyssinus group 1 major allergen T-cell fusion epitope peptide vaccine TAT-IhC-DPTCE against allergic asthma. Methods One hundred and twenty SPF-grade BALB/c mice were randomized into PBS group（group A）, asthma group （group B）, and immune treatment groups respectively receiving intraperitoneal（i.p.） injections of ProDer p 1 allergen（group C）, DPTCE （group D）, TAT-DPTCE（group E） or TAT-IhC-DPTCE（group F） （n=20 in each group）. In detail, PBS （group A） or allergen extract derived from Dermatophagoides pteronyssinus （groups B-F, 10 μg） was intraperitoneally injected on days 0, 7 and 14, and was continued by aerosol inhalation from day 21 for 7 consecutive days （0.5 μg/ml, once/day, 30 min each time）. The mice in groups C-F received i.p. injections of 100 μg/ml ProDer p 1, DPTCE, TAT-DPTCE and TAT-IhC-DPTCE respectively 30 min prior to inhalation challenge on days 25-27 as a specific immunotherapy, while those in groups A and B received 200 μl PBS. Twenty-four hours after the last inhalation challenge, all the mice were sacrificed. The lung histopathological changes were examined by HE staining. The levels of IFN-γ, IL-13, IL-10 and TGF-β in the bronchoalveolar lavage fluid（BALF） was determined with ELISA, and eosinophils in the BALF were counted（n=20 mice in each group）. The serum level of IgE, IgG1 and IgG2a in orbital blood was determined by ELISA（n=5 mice in each group）. Results HE staining revealed increased BALF eosinophils and decreased pulmonary inflammation in group F compared with group B. The IFN-γ level in group F [（298.75±26.09） pg/ml] was significantly higher than those in groups B[（158.71±20.89） pg/ml], C[（210.38±18.92） pg/ml], D [（229.44±13.00） pg/ml] and E[（233.24±20.39） pg/ml] （all P〈0.01）. Similar results were also found for IL-10 and TGF-β, while the IL-13 levels in groups C [（47.35±4.71） pg/ml], D [（41.90±4.28） pg/