中文摘要：

目的探讨害嗜鳞螨Ⅱ类变应原Lep d2对尘螨致敏哮喘小鼠的特异性免疫治疗效果。方法将30只SPF级BALB/c小鼠随机分为PBS组、哮喘组、Lep d2治疗组,于实验开始时及实验第7、14天用尘螨提取液对哮喘组及Lep d2治疗组小鼠腹腔注射致敏,第21天开始雾化吸入激发,连续7 d,第25-27天开始,Lep d2免疫治疗组于雾化前30 min用Lep d2变应原进行免疫治疗,PBS组及哮喘组用PBS进行腹腔注射及雾化吸入。最后1次雾化激发24 h后,收集支气管肺泡灌洗液（BALF）和血清,并进行脾细胞培养。采用HE染色观察各组小鼠肺组织病理变化;采用ELISA法测定BALF和脾细胞培养液上清中IFN-γ、IL-5、IL-13及血清中特异性Ig E（s Ig E）、s Ig G2a抗体水平。结果哮喘组及Lep d2治疗组小鼠出现哮喘急性发作症状,PBS组未出现该症状。哮喘组肺部炎性细胞浸润明显,PBS组及Lep d2治疗组肺部炎症较轻。Lep d2治疗组BALF和脾细胞培养液上清中IFN-γ浓度均高于哮喘组（P均〈0.01）,但IL-5和IL-13浓度则均低于哮喘组（P均〈0.01）。Lep d2治疗组s Ig E抗体水平低于哮喘组（P〈0.01）;但s Ig G2a抗体水平高于哮喘组（P均〈0.01）。结论害嗜鳞螨Ⅱ类变应原Lep d2疫苗可有效减轻哮喘小鼠肺部炎症。

英文摘要：

Objective To assess the effect of Lep d2 from Lepidoglyphus destructor as a vaccine for specific immunotherapy on murine model of asthma. Methods Thirty BALB/c mice（SPF）were randomly categorized into a PBS group,an asthma group,and a Lep d2 SIT group. The mice in the asthma group and Lep d2 SIT group were sensitized by intraperitoneal injection with extracts of dust mites on Days 0,7th,and 14 th,while those in the PBS group were injected with PBS. From the 21 st day,the asthma group and Lep d2 SIT group exposed to the extracts of dust mites were stimulated by aerosol inhalation for 7 successive days. During the period of the 25th-27 th Day,the mice in Lep d2 SIT group were injected intraperitoneally with Lep d2 allergen for SIT 30 min before nasal inhalation,whereas the PBS group and asthma group were treated with only PBS. Twenty-four hours after the final inhalation,all the mice were sacrificed,the bronchoalveolar lavage fluids（BALFs）were collected. The levels of IFN-γ,IL-5 and IL-13 in the BALF and the supernatant of splenocyte culture solution（SSCS）as well as the levels of specific Ig E（s Ig E）and s Ig G2 ain the sera were detected by ELISA. The lung tissues of the mice in the above 3 groups were stained by haematoxylin and eosin（HE）and observed by a microscope. Results The symptoms of acute asthma attack were observed in the mice of the asthma group and Lep d2 group,but not in the PBS group. The allergic inflammation changes in lung in the Lep d2 SIT group were significantly alleviated compared with those in the asthma group. The concentrations of IFN-γ in BALFs and SSCS of the mice in the Lep d2 SIT group were significantly higher than those in the asthma group（both P〈 0.01）,while the levels of IL-5 and IL-13 in the former group were significantly lower than those in the latter group（all P〈 0.01）. Meanwhile,the level of s Ig E of mice in the Lep d2 SIT group was significantly lower than those in the asthma group（P〈 0.01）,while the level of s Ig G2 aof mice in the form