中文摘要：

目的探讨粉尘螨1类变应原（Pro Der f 1）两种酶解产物（木瓜蛋白酶水解产物、胰蛋白酶水解产物）对过敏性哮喘特异性免疫治疗的效果。方法 50只6~8周龄的SPF雌性小鼠随机分为磷酸盐缓冲液（PBS）组、哮喘组、Pro Der f 1蛋白治疗组、木瓜蛋白酶水解产物免疫治疗组和胰蛋白酶水解产物免疫治疗组5组,每组各10只。用纯化的Pro De rf1蛋白于第0、7、14天腹腔注射致敏小鼠,第21天开始雾化吸入激发、连续7 d。各免疫治疗组于第25~27天雾化前30 min分别用Pro De rf1蛋白和其酶解产物进行特异性免疫治疗,PBS组用PBS进行腹腔注射和雾化激发。最后一次雾化激发后24 h内,各组小鼠引颈处死。观察各组小鼠肺组织病理变化,ELISA测定支气管肺泡灌洗液（BALF）和脾细胞培养上清中IL-4、IL-10、IL-17和IFN-γ含量及血清特异性抗体Ig E、Ig G2a水平。结果各免疫治疗组对Pro Der f 1哮喘小鼠进行免疫治疗后,肺组织切片观察表明：酶解产物治疗组和Pro Der f 1蛋白治疗组变态反应性炎症较哮喘组显著减轻,且炎性细胞浸润较哮喘组也显著减少,气道上皮结构与PBS组相近。Pro Der f 1蛋白治疗组、木瓜蛋白酶水解产物免疫治疗组、胰蛋白酶水解产物免疫治疗组和哮喘组小鼠BALF中的IL-4含量分别为（231.61±11.73）、（206.20±14.33）、（200.44±9.34）、（299.68±12.46）pg/ml,IL-10含量分别为（361.87±13.62）、（376.27±20.57）、（413.57±12.98）、（171.28±19.79）pg/ml,IL-17含量为（142.12±5.01）、（128.27±5.34）、（130.79±6.30）、（273.59±11.56）pg/ml,IFN-γ含量为（229.60±11.32）、（269.13±11.98）、（282.25±19.65）、（147.76±11.36）pg/ml;各治疗组和哮喘组小鼠脾细胞分泌因子IL-4含量分别为（218.54±12.62）、（220.21±10.73）、（201.59±18.54）、（256.86±15.53）pg/ml,IL-10含量分别为（360.45±13.10）、（383.41±19.81）、（413.51±13.14）?

英文摘要：

Objective To investigate the enzymatic hydrolysates of Pro Der f 1and their hydrolytic products for specific immunotherapy. Methods The asthma models of mice made by Pro Der f 1 allergen were treated by using two kinds of hydrolysates as vaccine for analyzing their effects of immunotherapy. Fifty female BALB/c mice were randomly divided into 5 groups（n =10 for each）,i.e.,a PBS group,an asthma group,an immunotherapy group by Pro Der f 1 protein,an immunotherapy groups by papain hydrolysates and trypsin hydrolysates. On day 0,7 and 14,the mice were intraperitoneally injected with 10 μg of Pro Der f 1 allergen,which was dissolved in 100 μl PBS containing 2%（W/V）Al（OH）3suspension. At day 21,the animals were caged in the airway challenge apparatus,and challenged by nebulized inhalation of allergen suspension（0.5 μg/ml）for 30 min for 7successive days. The mice were undergone allergen specific immunotherapy（ASIT）by intraperitoneal injection and of the immunotherapy group by Pro Der f 1 protein,immunotherapy groups by papain hydrolysates and trypsin hydrolysates（100 μg/ml）in a dose of 200 μl of reactive allergens,30 min prior to the inhalation treatment at day 25,26 and 27,respectively. The PBS group was managed with both intraperitoneal injection and aerosol of PBS. Twenty-four hours after the last challenge,all the mice were sacrificed. The bronchoalveolar lavage fluid（BALF）and sera were collected,and the splenocytes were cultured. The levels of IL-4,IL-10,IL-17 and IFN-γ in BALF and supernatant of splenocytes cultured（SSCC）were detected by ELISA,and the serum levels of specific Ig E and Ig G2 aantibodies were also detected by ELISA. Results Compared with the asthma group,the histologic examination of the lungs taken from the mice in the immunotherapy group by Pro Der f 1 protein,immunotherapy groups by papain hydrolysates and trypsin hydrolysates showed alleviated peribronchial and perivascular inflammatory infiltration,absently of eosinophils. The normal lung architectures were a