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中文摘要：
目的观察高迁移率族蛋白B1（HMGB1）对小鼠腹腔巨噬细胞凋亡的影响。方法分离培养小鼠腹腔巨噬细胞,经不同浓度的HMGB1（10、100、1000、10000ng/ml）作用24h,或以10000ng/ml的HMGB1作用不同时间（6、12、24、48h）后,以连接素Ⅴ/放线菌素D双染法,采用流式细胞术和激光扫描共聚焦显微镜检测巨噬细胞凋亡情况。结果经不同浓度的HMGB1刺激24h,其中10000ng/ml组HMGB1诱导巨噬细胞凋亡的作用最强,为（39.84±5.98）%,与对照组（4.49±1.72）%比较,差异具有显著性（P〈0.01）。以10000ng/ml的HMGB1作用巨噬细胞不同时间,其中24h组凋亡率发生最高,为（38.30±6.95）%,显著高于对照组（P〈0.01）。结论HMGB1可诱导小鼠腹腔巨噬细胞凋亡。
英文摘要：
Objective To investigate the effect of high mobility group box-1 protein （ HMGB1 ） on apoptosis of macrophages in mice. Methods The peritoneal macrophages were obtained from female BALB/c mice. After exposure to different doses of HMGB1 （10, 100, 1 000, and 10 000 ng/ml） for 24 hours, or exposure to 10 000 ng/ml for different time （6, 12, 24, and 48 hours）, cells were stained with Annexin V-PE and 7-amino-actinomycin-D （7-AAD）, then determined by flow cytometry and laser scanning confocal microscopy, respectively. Results Exposed to HMGB1 for 24 hours resulted in a dose-dependent induction of apoptosis, with increased levels of apoptosis observed at 100, 1 000, and 10 000 ng/ml compared with controls [（12.91 ±3.89）%, （18.76±3.41）%, and （39.84±5.98）%, respectively, vs. （4.49±1.72）%]. Exposed to HMGB1 at 10 000 ng/ml for 6, 12, 24, or 48 hours showed a time-dependent increase in apoptosis rate [ （18.33 ±4.60）%, （25.02 ±5.27）%, （38.30 ±6.95）%, and （21.81 ±7.96）% ]. The percentage of apoptotic cells markedly increased with a peak value at 24 hours after HMGB1 stimulation （ P 〈 0. 01 ）. Conclusion HMGB1 has a proapoptotic effect on murine peritoneal macrophages.
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