中文摘要：

本文在前期工作基础上,研究了两种海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met、Val-Thr-Pro-Tyr的抗氧化活性。应用电子自旋共振的方法（ESR）,检测海参肠自溶寡肽对羟基自由基（·OH）、超氧阴离子自由基（O2-·）、1,1-二苯基-2-三硝基苯肼（DPPH·）自由基的清除情况。同时,采用人白血病细胞Jurkat,考察海参肠自溶寡肽对H2O2诱导的细胞氧化损伤的影响。结果表明,两种海参肠自溶寡肽对·OH、O2-·和DPPH自由基均有很强的清除能力,且呈明显的量效关系。对于不同自由基,Val-Gly-Thr-Val-Glu-Met在相应的浓度下（·OH自由基-1和5 mM、O2-·自由基-1、5和20 mM、DPPH自由基-1和8 mM）清除能力均显著高于Val-Thr-Pro-Tyr（p〈0.05）。海参肠自溶寡肽在浓度为5 mM时未能对H2O2诱导的Jurkat细胞DNA氧化损伤起到保护作用,而浓度为20 mM时引起Jurkat细胞死亡。这些结果说明,海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met和Val-Thr-Pro-Tyr具有自由基清除能力,具有一定的开发价值。

英文摘要：

In this paper,the antioxidant activities of oligopeptides（Val-Gly-Thr-Val-Glu-Met and Val-Thr-Pro-Tyr） from sea cucumber guts were studied by measuring the scavenging abilities on hydroxyl radical,superoxide radical and 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical using an ESR spectrometer.Moreover,the influence of oligopeptides on H2O2-induced oxidative damage in Jurkat cells was also investigated.The results showed that two kinds of oligopeptides from sea cucumber guts showed scavenging abilities on ·OH,O2-· and DPPH· in a dose-dependent manner.The scavenging ability of Val-Gly-Thr-Val-Glu-Met was significantly higher than that of Val-Thr-Pro-Tyr at different concentrations（p〈0.05）.The oligopeptides failed to protect the DNA oxidative damage induced by H2O2 at concentrations of 5 mM in Jurkat cells,while induced cell death at concentration of 20 mM.These results suggest that the oligopeptides from sea cucumber guts（Val-Gly-Thr- Val- Glu-Met,Val-Thr-Pro-Tyr） possess free radical scavenging abilities,and have certain development value.