中文摘要：

对海参乙酰胆碱酯酶（AChE）粗酶的酶学性质进行研究。采用含0.1%Triton X-100的PBS缓冲液（0.1 M,pH7.4）分别从海参肠和海参体壁中提取AChE,配合Ellman法测定AChE酶活力。结果表明,海参肠与体壁AChE具有相似的酶学性质,其最适反应pH值为8.0,pH在6~8时稳定;最适反应温度为35℃左右,在25~45℃内热稳定性较好。海参乙酰胆碱酯酶粗酶液能有效水解碘化硫代乙酰胆碱（AcSChI）,但对碘化硫代丁酰胆碱（BuSChI）的作用较弱。当底物AcSChI浓度大于50 mM时产生明显的底物过量抑制效应。金属离子Sn2＋、Zn2＋、Hg2＋、Ag＋、Cr6＋、Cu2＋及毒扁豆碱和BW284c51均对海参肠和海参体壁乙酰胆碱酯酶有不同程度的抑制作用。

英文摘要：

The enzymic characteristics of crude acetylcholinesterase（AChE） from sea cucumber were studied.The crude AChE was extracted from the gut or body wall of sea cucumber with PBS buffer containing 0.1% Triton X-100,and the activity of AChE was determined by the method of Ellman.The results indicated that AChEs from the gut and body wall of sea cucumber have the similar enzymatic characteristics.The crude AChE displayed maximum activity at pH 8.0 and over 35 ℃,and showed high stability in the range of pH 6.0～8.0 and favorable temperature 25～45 ℃.AChE from sea cucumber hydrolyzed acetylthiocholine iodide（AcSChI） effectively,but showed little effect on butyrylthiocholine iodide（BuSChI）.At a concentration higher than 50 mM,AcSChI inhibited the enzyme activity obviously.The activity of AChE was inhibited by Sn2＋,Zn2＋,Hg2＋,Ag＋,Cr6＋,Cu2＋and some inhibitors such as Eserine and BW284c51.