中文摘要：

以3-羧基苯基-乙基二甲基铵为配基,与溴化氰活化琼脂糖凝胶交联制备亲和层析柱,分离海参肠中乙酰胆碱酯酶（acetylcholinesterase,AChE）,对其洗脱条件进行优化。结果显示：其适宜平衡体系为含0.3mol/L NaCl的PBS（0.05mol/L,pH7.4）缓冲溶液,适宜洗脱体系为含0.2mol/L四乙基碘化铵的PBS（0.05mol/L,pH7.4）缓冲溶液。此条件下,海参肠AChE可得到较好的吸附和分离,初步纯化后的AChE经Native-PAGE电泳分离出3条具有酶活性的条带,说明AChE在海参肠内可能以多种形式存在,呈现多态性。

英文摘要：

An affinity chromatography column was prepared by cross-linking of 3-carboxyphenyl ethyldimethyl ammonium（CEA） as a ligand with CNBr-Sepharose-4B and used for an investigation into the optimization of elution conditions for affinity chromatography of acetylcholinesterase（AChE） from the gut of sea cucumber（Stichopus japonica）.The appropriate balance system was PBS（0.05 mol/L,pH 7.4） containing 0.3 mol/L NaCl,and the appropriate elution system was PBS（0.05 mol/L,pH 7.4） containing 0.2 mol/L tetraethyl ammonium iodide.Under these conditions,AChE was well adsorbed and separated.Three active bands were separated in the native-PAGE pattern of Preliminarily purified AChE,AChE in the gut of sea cucumber may have different forms and display polymorphism.