中文摘要：

分别利用PCR-琼脂糖凝胶电泳和PCR-RFLP方法对皖南黑猪、皖南花猪、定远猪、安庆六白猪4个安徽地方猪种共213头繁殖母猪进行FSHβ和ESR基因多态性及其与产仔性状的关系进行研究,旨在为安徽地方猪种的标记辅助选择提供科学依据。结果表明：（1）ESR和FSHβ基因在4个地方猪种中都存在多态性,ESR在安庆六白猪、定远猪中出现了AA、AB、BB3种基因型,而在皖南黑猪、皖南花猪中只出现了AA、AB 2种基因型;FSHβ在皖南黑猪、皖南花猪中出现了CC、CD、DD 3种基因型,而在安庆六白猪、定远猪中只出现了CC、CD 2种基因型;（2）不同基因型母猪的TNB、NBA存在一定差异,ESR不同基因型对定远猪和皖南花猪经产母猪的TNB、NBA影响显著（P〈0.05）;FSHβ不同基因型对皖南黑猪初产、经产和定远猪经产母猪的TNB、NBA影响显著（P〈0.05）。以上结果表明ESR和FSHβ基因不同基因型对部分安徽地方猪的产仔性状有着一定的影响,可作为其标记辅助选择的潜在遗传标记。

英文摘要：

To provide basic information for marker-assisted selection in pig breeding, the association of ESR and FSHβ genes with litter size in 213 local breeds of Wannanhei, Wannanhua, Dingyuan and Anqingliubai pigs was analyzed using PCR-AGE and PCR-RFLP methods. The results showed that polymorphisms of ESR and FSHβ genes in four local pig breeds existed. Three ESR genotypes（AA, AB and BB） were detected in Anqingliubai and Dingyuan pigs, while only AA and AB genotypes of ESR were detected in Wannanhei and Wannanhua pigs. For the FSHβ gene, CC, CD and DD genotypes were found in Wannanhei and Wannanhua pigs, while CC and CD genotypes were detected in Anqingliubai and Dingyuan pigs. The results also indicated that pigs with different genotypes had different TNB and NBA. In Dingyuan and Wannanhua pigs, the TNB and NBA of the multiparous sows were significantly affected by different genotypes of ESR（P〈0.05）. In Wannanhei and Dingyuan pigs, the TNB and NBA of the sows were significantly affected by different genotypes of FSHβ（P〈0.05）. Therefore, the association of ESR and FSHβ genotypes with litter size of four local pig breeds in Anhui could provide useful information for marker-assisted selection in pig breeding.