采用TAS-ELISA,DAS-ELISA和ID-ELISA 3种检测方法,选用11种病毒病原血清对禄劝烟区烟草成熟期受害典型样本进行检测.结果表明,禄劝烟区烟草成熟期的病毒病具有从单一侵染向复合侵染演变趋势,且病原危害种类以TMV为主,CMV次之,除此之外尚存在TEV,TVBMV等病毒.烟草成熟期受害叶片症状呈现"复杂化与多样化"特征,借助先进检测手段、扩大病原血清种类是探索清楚当地烟草成熟期主要病毒病种类的必要措施.
By using the three detection methods of TAS-ELISA,DAS-ELISA and ID-ELISA,10 kinds of pathogenic virus serum were selected for testing typical disease symptom leaf samples from tobacco leaves mature stage in Luquan county.The results showed that the tobacco virus diseases had a trend from a single infection to mixed infection at Luquan tobacco mature period,and TMV was main pathogenic,CMV secondly.In addition,TEV,TVBMV and others existed.The symptoms for tobacco leaves infected by virus diseases appeared complex and diverse feature.With the help of advanced detection means and increasing pathogenic serum types will be necessary measures to find out the main virus types during tobacco mature stage in Luquan County.