中文摘要：

探讨维药异常黑胆质成熟剂（ASM）抗肿瘤作用及其对肿瘤细胞迁移的影响。体外培养人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株,采用四甲基偶氮唑蓝（tetrzolium-based colorimetric assay,MTT）法检测不同浓度ASM（1、2.5、5、10、15、20、50mg/mL）对人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株4种肿瘤细胞增殖的影响;选择人肝癌Bel-7402细胞株为研究对象,采用细胞划痕运动实验和扫描电镜技术,观察不同浓度ASM对肝癌细胞迁移的影响。MTT检测结果显示,ASM对人肝癌Bel-7402细胞株、人乳腺癌BCAP细胞株、人宫颈癌Hela细胞株、人胃癌BGC-823细胞株生长均有一定抑制作用,且在15～20mg/mL作用下最为明显,对不同肿瘤细胞株抑制作用不一;体外细胞划痕运动实验和扫描电镜结果显示,ASM对人肝癌Bel-7402细胞运动有一定抑制作用。由此推论,ASM对肿瘤细胞增殖和肿瘤细胞迁移有一定抑制作用。

英文摘要：

The possible mechanism of Uighur medicine Abnormal Savda Munzip （ASM） on the anti-tumor effects and its influence on tumor cell migration are explored. MTT was applied to determine the cell proliferation of human hepatoma （Bel-7402）,human breast carcinoma （BCAP）,human cervical cancer （Hela）,and human gastric cancer proliferation （BGC-823） after treated with different concentration of ASM （1,2.5,5,10,20,50mg/mL） for 48h; Human hepatoma cell line （Bel-7402） was selected for the cell motility study,the cell migration influence on human hepatoma （Bel-7402） were assessed by the wound-healing assay and the Scanning Electron Microscopy （SEM） technique after treated with ASM （5,10,20,50mg/mL） for 48h. ASM produced the cytotoxinic effect on these four type of tumor cells in vitro and all type cells showed the obviously vitro inhibition after treated with ASM （15,20mg/mL）,there were difference inhibited effect among the four type of tumor cells,the results of wound-healing assay with SEM show that the moving ability of Bel-7402 treated with ASM has decreased. It is deduced that ASM might inhibit the ability of proliferation and moving of tumor cell.