中文摘要：

由于原核细胞mRNA3′端不存在ploy（A）结构，因而原核细胞DDRT-PCR引物设计不同于真核细胞。尽管不能根据oligo（dT）设计引物，但利用全基因中高度分散重复的短序列或回文序列却能有效地克服mRNA分子结构影响，最大限度地扩增全长cDNA。并且这两种引物设计方法还可以提高RNA指纹图谱的重复性，降低反应的假阳性，为原核细胞DDRT-PCR引物设计提供新的思路。

英文摘要：

Due to the lack ofpolyadenylation in prokaryotic mRNA, the primer design ofprokaryotic DDRT-PCR is different from eukaryocyte. Oligo （dT） primers are effectively replaced with primers designed according to highly iterated palindrome in complete genome. The primer design method can not only overcome shortcoming in prokaryotic mRNA to maximatily amplify whole cDNA, but also enhance the RNA finger-printing reproducibility and weaken false positive.It supples novel thought for prokaryotic differential display RT-PCR.