登革病毒(dengue virus,DENV)是引起登革热(dengue fever,DF)的病原体,经蚊叮咬而传播疾病.DF主要流行于热带和亚热带地区.目前,DF已经发展成一个日益严重的全球性公共卫生问题.2012年,DF被列为全球最重要的蚊虫传播性疾病.快速而准确的针对DENV感染的检测技术能够有效地控制DF的爆发及减少死亡病例.目前DENV感染的检测技术主要有病毒分离培养、分子生物学诊断及血清学诊断三类,三类检测技术各有优缺点,应该根据具体情况选用适当的检测手段.
Dengue virus (DENV) is the pathogen of Dengue fever (DF), which is a major public health prob- lem in tropical and sub-tropical regions and the most important arboviral disease of humans. In 2012, DF ranks as the most important mosquito-borne viral disease in the world. Fast and accurate diagnosis for DENV infection can effectively control the outbreak of DF and reduce death cases. Methods used for diagnosis of dengue infections include virus isolation, serology and molecular techniques. Each of three methods has its advantages and disadvantages, the suitable choice of methods in clinical practice should based on the clini- cal condition of patients.