中文摘要：

目的探讨IL-4基因启动子区SNP-1098T〉G和-590C〉T位点与HCV慢性感染的相关性.方法采用Taq Man探针基因分型方法对HCV慢性感染患者192例和健康体检人群170例IL-4基因启动子SNP-1098T〉G和-590C〉T位点进行基因分型,计算2个SNP位点的连锁不平衡,并构建单倍型,获得以上2个SNP位点及其单倍型与HCV慢性感染的相关性数据.结果 IL-4基因启动子SNP-1098T〉G和-590C〉T位点基因型和等位基因频率分布差异在病例组和对照组中无统计学意义（P〉0.05）.连锁不平衡结果显示,SNP-1098T〉G和-590C〉T位点为连锁不平衡,D’值为0.937.构建单倍型后发现,SNP-1098T〉G和-590C〉T位点构建的单倍型频率分布差异在病例组和对照组中无统计学意义（P〈0.05）.结论 IL-4基因启动子区SNP-590C〉T和-1098T〉G多态性与云南汉族人群HCV慢性感染的无相关性.

英文摘要：

Objective To study the relationship between the allele polymorphisms SNP-1098T 〉G and -590C 〉T in interleukin-4 gene promoter and HCV chronic infection in Han population in Yunnan province . Methods Two single nucleotide polymorphisms （SNPs） in the IL-4 promoter region, -1098T〉G and -590C 〉T in 192 HCV chronic infected patients and 170 healthy person were determined by real-time TaqMan polymerase chain reaction. We calculated the linkage disequilibrium of the two SNPs, and then constructed haplotypes, obtained correlative data between SNP-1098T 〉G, -590C 〉T and HCV chronic infection, and the correlation between haplotype frequency and HCV chronic infection. Results IL4-1098T 〉G and -590C 〉T genotype and haplotypes frequency showed no significant association with HCV infected patients and healthy person （P〉0.05） There was linkage disequilibrium of IL-4 gene between SNP-1098T〉 G and -590C 〉T. The frequency of haplotypes constructed by SNP-1098T〉G and -590C 〉T showed no significant difference between HCV infected patients and healthy person （P 〉0.05） Gonclu sion SNPs-1098T 〉G and -590C 〉T in IL-4 gene promoter has no association with chronic HCV infection in Yunnan Han population.