中文摘要：

探讨芍药苷（paeoniflorin,Pae）对佐剂性关节炎（adjuvant arthritis,AA）大鼠血清抗体水平和脾脏淋巴细胞cAMP水平的影响。大鼠足跖皮内注射弗氏完全佐剂（FCA）诱发大鼠AA模型,聚乙二醇（PEG）6000法检测血清循环免疫复合物（circulating immune complexes,CIC）;酶联免疫吸附（ELISA）法检测血清中抗C II抗体（anti-C IIantibody）水平和抗结核杆菌抗体（anti-TB antibody）水平;放射免疫法（RIA）检测芍药苷体外给药对脾细胞中cAMP水平的影响。大鼠免疫后第17天开始分别灌胃给予芍药苷（25、50及100 mg·kg^-1）和雷公藤多苷（40 mg·kg^-1）对照药,连续给药7 d。芍药苷（50及100 mg·kg^-1）可明显降低AA大鼠血清中升高的CIC水平、抗C II抗体水平和抗结核杆菌抗体水平,芍药苷组对AA模型组的抑制率（inhibition ratio,IR）与剂量呈正相关;芍药苷体外给药（12.5、62.5及312.5 mg·L^-1）使AA大鼠脾细胞升高的cAMP水平降低。芍药苷治疗后,明显降低AA大鼠的血清抗体水平及大鼠脾细胞升高的cAMP水平。

英文摘要：

This study is to investigate the effects of paeoniflorin （Pae） on the levels of related serum antibodies and cAMP of splenocytes in rats with adjuvant arthritis. Complete Freund＇ s adjuvant was used to induce AA in rats. The level of circulating immune complexes in serum was determined by PEG6000 assay, and the levels of anti-C II antibody and anti-TB antibody in serum were measured by enzyme-linked immunosorbent assay （ELISA） , the level of cAMP in splenocytes was measured by radioimmunoassay, separately. Pae （25, 50, and 100 mg·kg^-1） and GTW （40 mg·kg^-1） were given by intragastric administration for 7 days from the 17th day after immunization. Pae （50 and 100 mg ·kg^-1 ） reduced the levels of circulating immune complexes, anti-C II antibody and anti-TB antibody in serum in rats with adjuvant arthritis. The inhibition ratios of Pae groups to AA model group were dosage-dependent; Pae （ 12.5, 62.5, and 312.5 mg ·L^-1 ） decreased the elevated levels of cAMP in splenocytes in vitro. Pae （ig） decreased the levels of related serum antibodies and elevated the level of cAMP in rats with adjuvant arthritis.