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中文摘要：
目的探讨适合人成纤维样滑膜细胞（FLS）低温冷藏保存的最佳冻存时间。方法将FLS经-80℃冰箱冷冻保存。在冷冻后2、3、4、5wk分别复苏，培养。观察FLS的生长情况和细胞存活率及24h贴壁率。结果冻存5wk的FIN细胞存活率（66±1．3）％，较冻存2wk（90±2．0）％有明显下降；冻存5wk的FLS24h贴壁率（61．3±2）％，与冻存2wk（85．9±3）％比较有明显下降；未冻存和冻存2wk复苏后FLS的增殖能力无明显差异。结论FLS可以进行低温冷冻，冻存时间4wk为宜，
英文摘要：
Aim To determine appropriate length of cryopreservation time for fibroblast-like synoviocytes. Methods FLS were cryopreserved in - 80℃ refrigerator, resuscitated and cultured after being frozed for 2,3,4 and 5 weeks. The cell viability and 24 hours adherence rate were evaluated. Results There was significant difference in the rate of cell livability of FLS cryopreserved for 5 weeks compared with that for 2 weeks. Significant difference was also observed in the 24 hours adherence rate of FLS cryopreserved for 5 weeks compared with that for 2 weeks. There was no difference in the proliferation of FLS routinely maintained compared with those cryopreserved for 2 weeks. Conclusions FLS can be cryopreserved and the time limit of cryopreservation should be 4 weeks.
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