中文摘要：

目的探讨在不同免疫状态下，芍芪多苷（Shaoqiduogan，SQDG）对迟发型变态反应（DTH）小鼠细胞免疫功能的作用。方法采用2，4-二硝基氯苯（DNCB）诱导小鼠DTH模型及环磷酰胺（Cy）诱导小鼠免疫低下或亢进模型。SQDG予小鼠连续灌胃7d。MTT法检测ConA诱导的小鼠胸腺T淋巴细胞增殖，小鼠胸腺细胞增殖法检测ConA诱导的小鼠胸腺T淋巴细胞培养上清中自细胞介素2（IL-2）的活性。结果在小鼠DTH模型，SQDG（120mg·kg^-1）可降低耳肿胀、胸腺指数、ConA诱导的胸腺T淋巴细胞增殖反应以及ConA诱导的胸腺T淋巴细胞培养上清中IL-2的活性。DNCB初次致敏当日腹腔注射（ip）Cy（150mg·kg^-1）可以造成DTH低下模型，致敏前3dipCy（250mg·kg^-1）可造成DTH亢进模型；SQDG（60和120mg·kg^-1）能明显上调DTH低下模型小鼠耳肿胀、ConA诱导的小鼠胸腺T淋巴细胞增殖及小鼠胸腺T淋巴细胞培养上清中IL-2的水平；且能明显下调DTH亢进模型小鼠上述指标的水平。结论SQDG对小鼠细胞免疫具有双向调节的作用。

英文摘要：

Aim To investigate the effects of Shaoqiduogan（SQDG） on cellular immunity in mice with delayed-type hypersensitivity （ DTH ）. Methods DTH model induced by 2, 4-dinitrochlorobenzene （ DNCB ）and cyclophosphamide（Cy） suppressed and potentiated DTH model were used. SQDG（30, 60 and 120 mg· kg^-1 ） were given intragastrically （ ig ） once daily for 7 consecutive days. Concanavalin A（ Con A）-induced Tcells proliferation was measured by MTT assay. The activity of IL-2 in culture supernatants of Con A-induced T cells proliferation was measured by testing its ability to support the proliferation of Con A stimulated thymocytes which was detected by MTT assay. Results In mice with DTH, SQDG（ 120 mg· kg^-1 ）reduced ear swelling, thymus index, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of Con A-induced T cells proliferation. The suppression and potentiation of DTH in mice were induced by given an intraperitoneal （ ip ） injection of Cy at the dose of 150mg· kg^-1 on primary sensitization day and 250 mg· kg^-1three days before primary sensitization. SQDG（60 and 120 mg· kg^-1） upregulated ear swelling, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of T cells from mice with Cy-suppressed DTH; and downregulated levels of the aforementioned indexes in mice with Cy-potentiated DTH. Conclusion SQDG possesses a dual modulatory effect on cellular immunity in mice.