采用硫酸铵沉淀及凝胶过滤层析方法,纯化花生主要过敏原蛋白Ara h1,通过聚丙烯酰胺凝胶电泳(SDS-PAGE)结合免疫印迹实验(Western-Blotting)进行鉴定。结果表明:可纯化出纯度90%以上的Ara h1过敏原蛋白。
In order to obtain the peanut allergen Ara h1,ammonium sulfate precipitation and size exclusion chromatography were used for the purification.The identification of peanut allergens was carried out using SDS-PAGE and Western blot analysis.The results indicated that peanut allergen Ara h1 was isolated with purity of more than 90%.