中文摘要：

目的探讨双歧杆菌对食物过敏的治疗作用。方法 4周龄棕色挪威大鼠（Brown-Norway Rat,BN Rat）构建卵清蛋白（ovalbumin,OVA）致敏动物模型,以OVA 1 mg每天连续灌胃6周,同时分别建立PBS对照组和双歧杆菌治疗组;第6周取血测定血清中OVA特异性IgE（OVA-IgE）水平和IgG（OVA-IgG）水平作为模型评测指标;采用张力换能器测定肠道收缩情况,苏木素-伊红染色（Hematoxylin-Eosin stain,HE）及甲苯胺蓝（Toluidine blue Benzen esulfonic acid,TB）染色观察肠组织病理变化和肠粘膜肥大细胞数目和形态变化。结果第6周时实验组OVA-IgE和OVA-IgG含量显著升高,与对照组相比,OVA-IgE存在显著性差异（P〈0.05）,OVA-IgG存在极显著差异（P〈0.01）;经益生菌治疗后,OVA-IgE和OVA-IgG含量显著下降,与实验组相比,存在显著性差异（P〈0.05）。双歧杆菌治疗组肠组织收缩较OVA组显著降低,存在显著性差异（P〈0.05）;HE染色和甲苯胺蓝染色可见双歧杆菌治疗组肠粘膜破坏程度、肠粘膜肥大细胞脱颗粒现象较OVA组缓解。结论 OVA致敏模型和双歧杆菌治疗组模型成功建立,双歧杆菌对食物过敏有较好的治疗作用。

英文摘要：

To study the therapeutic effects of bifidobacterium on food allergy,4-week-old Brown-Norway Rats（BN） were sensitized by feeding ovalbumin 1 mg every day for 6 weeks to establish food allergy animal model,which were then randomly divided into 3 groups： PBS subgroup,OVA subgroup and bifidobacterium treatment subgroup.Sixth week after,blood of the rats were collected for detection serum OVA specificity IgE（OVA-IgE） level and OVA specificity IgG（OVA-IgG） level as allergic judgment standard;the contractility of isolated intestine sections was studied by transducers;the changes of intestinal tissue pathological and the intestinal mucosa mast cells were observed by Hematoxylin-Eosin（HE） stain and Toluidine blue Benzen esulfonic acid（TB） stain,respectively.OVA-IgE and-IgG levels significantly increased on the sixth week,as compared with the control subgroup（P 〈 0.05 and P 〈 0.01,respectively）.After treatment by bifidobacterium,the levels of OVA-IgE and-IgG decreased（P 〈 0.05）,the contractility of intestine tissue reduced significantly（P 〈 0.05）,and the damage of intestinal mucosa alleviated,and the degranulated mast cells decreased statistically,as compared with OVA subgroup.In conclusion,bifidobacterium have well therapeutical effects in food allergy treatment.