实时荧光定量PCR技术是一种新兴的核酸定量分析技术,与普通PCR定性分析相比,具有简单高效、准确灵敏、实时分析等更多的优势,是一项实用性很强的技术方法。文中主要对实时荧光定量PCR技术的操作原理、荧光类型及其在食品检测和科学研究领域中的应用进行综述。
Compared with conventional qualitative PCR,the real-time fluorogenic quantitative PCR has the characteristic of simple,efficient,accurate and sensitive.Real-time analysis is a very practical method.The purpose of this article is to summarize the principle and fluorescent systems of RTQ-PCR and its application in food detection and scientific research.