中文摘要：

为了研究羟基磷灰石（HA）纳米微粒对不同种类低温保护剂（质量浓度为60％的渗透性丙三醇和质量浓度为50％的非渗透性PEG-600）反玻璃化结晶的影响，利用低温显微镜研究了含有不同粒径（20nm、40nm、60nm）和不同质量浓度（0．1％、0．5％）HA纳米微粒的丙三醇和PEG-600溶液在反玻璃化过程中的结晶现象。实验结果表明：丙三醇和PEG-600溶液在反玻璃化过程中的冰晶形貌分别为枝状和球形，且形貌都不随添加纳米微粒而改变。与未添加纳米微粒的丙三醇溶液相比，添加60nm、0．5％纳米微粒溶液的析晶分数仅为未添加溶液的1／7。PEG-600溶液在-64℃～-52℃区间内的析晶分数显著增加，其中添加40nm、0．5％纳米微粒的PEG-600溶液的析晶分数增加了90％。冰晶的生长速率存在两个极大值现象，而添加40nm、0．5％纳米微粒的丙三醇溶液、未添加和添加60nm纳米微粒的PEG-600溶液的冰晶生长速率都只有一个极大值。

英文摘要：

To study the effects of Hydroxyapatite （HA） nanoparticles on devitrification of different cryoprotectant solutions, the crystalli- zation of Glycerol（ mass concentration is 60% ） and PEG-600（ mass concentration is 50% ） solutions with HA nanoparticles of different particle size（20nm,40nm ,60nm）and concentrations（0.1% ,0.5% ）were observed under a cryomicroscope. Experimental resuhs showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanopartieles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between - 64℃ and - 52℃, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nrn, and PEG-600 solution without nanoparticles.