中文摘要：

本文利用低温显微镜研究了含有不同粒径（20nm、40nm、60nm）浓度为0．5％HA纳米微粒的丙三醇溶液（40％，w／w）在不同温度变化速率（5℃／min、10℃／min、15℃／min）下的冻结和融化过程．试验结果表明：随着温度变化速率的增大，含60nm纳米微粒的丙三醇溶液，冰晶成核温度从-40℃降低到-50℃；含20nm纳米微粒的丙三醇溶液，冰晶融化起始温度从-70℃升高到-60℃。对于含40nm纳米微粒的丙三醇溶液，温度变化速率为10℃／min的冰晶属性差异显著．相比未添加、添加20nm和40nm纳米微粒的丙三醇溶液，添加60nm纳米微粒的丙三醇溶液无溶质分子淀析现象．

英文摘要：

The freezing and thawing of glycerol （40%）solutions with HA nanoparticles of different particle size （20nm, 40nm, 60nm） and concentrations（0. 5 %） were observed under a cryomicroscope at different cooling and war- ming rate（5 ℃/min, 10℃/min, 15 ℃/min）. Experimental results showed that the nucleation temperature of glycerol solution containing 60nm HA decreased from -40℃--50℃, and the incipient melting temperature of glycerol so- lution containing 20nm HA increased from --70℃- 60℃ with the speed-up of temperature change. The ice habits of glycerol solution containing 40nm HA at the velocity of 10℃/min is different remarkably. The glycerol solution containing 60nm HA has no precipitation of solute molecules when comparing with the others.