中文摘要：

为了研究羟基磷灰石HA纳米微粒对低温保护剂反玻璃化结晶的影响，本文利用DSC和低温显微镜研究了含有不同粒径（20nm、40nm、60nm）和不同质量浓度（O．1％、0．2g、0．4％、0．8N）HA纳米微粒的PEG-600（50％，w／w）溶液反玻璃化过程中的结晶现象．试验结果表明：与未添加纳米微粒的PEG-600溶液相比，加入40nm、0．4％纳米微粒的HA-PEG600溶液的反玻璃化温度升高了7℃;加入20nm、0．4％和40nm、0．8％纳米微粒的HA-PEG600溶液的冰晶生长速率分别降低了35％和提高了50％；纳米低温保护剂溶液的冰晶形貌从大圆形变成了小圆形、枝晶或小圆形中夹带枝晶．

英文摘要：

To study the effects of HA nanoparticles on devitrification of cryoprotectant solutions, The crystallization of PEG-600（50H）solutions with HA nanopartieles of different particle size（20nm.40nm. 60nm）and concentrations（0. 1%.0. 2%. 0. 4%. 0. 8%）were observed under a cryomicroscope, and vitrification parameters were measured by DSC. Experimental results showed that devitrifieation temperature of PEG-600 solution containing 0. 4H HA with the particle size of 40nm increased by 7℃, the growth rate of ice crystal of PEG-600 solution containing 0. 4% HA with the particle size of 20nm and 0. 8% HA with the particle size of 40nm decreased by 35% and increased by 50% respectively. The structure of ice crystal in PEG-600 solution with HA changed remarkably.