中文摘要：

将传统肝细胞冻存保护剂配方中的DMS0的浓度减为5％，并添加不同浓度的D-山梨醇、木糖醇或麦芽糖醇，置于-80℃的冰箱冻存两周，两周后将细胞快速复温，进行细胞存活率、24h贴壁率以及细胞形态学的检测，并与10％浓度DMso组对照比较。结果表明，D-山梨醇、木糖醇、麦芽糖醇均对人肝细胞的低温保存有一定的保护作用，其中5％DMSO＋0．4MD-山梨醇组复温后细胞的存活率、贴壁率均优于其他冻存液组，D．山梨醇与DMSO联合使用降低了冻存保护剂UDMSO的浓度，表明两者有协同作用。

英文摘要：

Different types of sugar alcohol is usually used as cryoprotective agents （CPAs） to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation.