Buthus martensii Karsch 昆虫毒素(BmK 信息技术) 基因多被插入到 Autographa californica 的染色体衣壳 nucleopolyhedrovirus (AcMNPV ) 到构造 recombinant baculovirus, AcMNPV-BmK 信息技术。BmK 信息技术的表示用 RT-PCR,点污点和 SDS 页分析被证实。对 Spodoptera exigua 幼虫的剂量致命的时间回答在野类型的 baculovirus AcMNPV 和 recombinant 病毒 AcMNPV-BmK 信息技术之间被比较。在 1 x 10 的集中(7 ) PIBs/mL, recombinant baculovirus 的中部的致命的时间(LT50 = 73.6 h ) 在第三中间形态 S 上。exigua 幼虫在野类型病毒的功效上显示出 13.2% 的改进(LT50 = 84.8 h ) 在 192 h 感染期间。
An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 10^7 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.