位置:成果数据库 > 期刊 > 期刊详情页
Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy
  • ISSN号:1001-6538
  • 期刊名称:科学通报(英文版)
  • 时间:0
  • 页码:1855-1860
  • 语言:中文
  • 分类:TQ453[化学工程—农药化工]
  • 作者机构:[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
  • 相关基金:Supported by the National Natural Science Foundation of China (Grant Nos. 30670282 and 30700534) and the Department of Science and Technology of Shanxi Province
  • 相关项目:纤毛虫核糖体蛋白L11在翻译终止中的作用
中文摘要:

Buthus martensii Karsch 昆虫毒素(BmK 信息技术) 基因多被插入到 Autographa californica 的染色体衣壳 nucleopolyhedrovirus (AcMNPV ) 到构造 recombinant baculovirus, AcMNPV-BmK 信息技术。BmK 信息技术的表示用 RT-PCR,点污点和 SDS 页分析被证实。对 Spodoptera exigua 幼虫的剂量致命的时间回答在野类型的 baculovirus AcMNPV 和 recombinant 病毒 AcMNPV-BmK 信息技术之间被比较。在 1 x 10 的集中(7 ) PIBs/mL, recombinant baculovirus 的中部的致命的时间(LT50 = 73.6 h ) 在第三中间形态 S 上。exigua 幼虫在野类型病毒的功效上显示出 13.2% 的改进(LT50 = 84.8 h ) 在 192 h 感染期间。

英文摘要:

An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 10^7 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.

同期刊论文项目
期刊论文 15 会议论文 9 获奖 2
同项目期刊论文