中文摘要：

目的探讨B淋巴细胞刺激因子（BLyS）及其受体在多发性骨髓瘤（MM）中的表达及其意义。方法采用流式细胞术（FCM）分析人MM肿瘤细胞系KM3及CZ-1细胞表面BLyS及其受体的表达；RT—PCR及Western blot进一步验证BLyS及其受体的表达；采用荧光免疫细胞化学法和激光共聚焦技术鉴定KM3细胞中BEyS蛋白的表达与定位；WST细胞增殖实验检测BLyS对MM肿瘤细胞生长与存活的影响。ELISA及荧光定量-聚合酶链反应（RTQ-PcR）测定MM患者BLyS蛋白与mRNA的表达水平。同时分析乳酸脱氢酶（LDH）、β2微球蛋白浓度与BLys蛋白及mRNA表达水平的关系。结果①MM细胞能够表达BLyS及其受体；②BLyS定位表达于KM3细胞的浆膜上；③BLyS促进MM细胞的生长与存活；④MM患者BLys蛋白或mRNA表达水平显著高于正常对照组（P值均〈0．01）；⑤直线相关分析显示LDH、β2微球蛋白浓度与BLyS蛋白及mRNA表达水平呈显著相关性。结论BLyS及其受体对于MM发生、发展可能起着重要的作用。

英文摘要：

Objective To investigate the expression of B lymphocyte stimulator （BlyS） and its receptors in multiple myeloma （MM） cells, and to explore the relationship between BLyS and the development of human multiple myeloma. Methods Flow cytometry, RT-PCR and Western blot were used to examine the expression of BLyS and its receptors in MM（ KM3 and CZ1 ） cells. Fluorescence immunocytochemical method and confocal laser scanning technique were applied to the localization of BLyS in KM3 cell. WST proliferation assay was used to examine the effect of BLyS on MM cells growth and survival. Linear correlation analysis was used to detect LDH and beta 2-microglobulin （132M） levels with BLyS protein and mRNA expressions in MM patients. Results ① BLyS and its receptors were expressed in MM cells. ②BLyS protein was localized on the KM3 plasma membrane. ③BLyS promoted survival and proliferation of MM cells. ④MM patients had significantly higher expression levels of BLyS [ 77.42% （ 24/31 ） ] BLyS mRNA [ 93.55 % （ 29/31 ） ], which were significantly correlated with the levels of LDH and beta 2-microglobulin （ 132 M）. Conclusion BLyS and its receptors in MM cell lines and MM patient bone marrow might have a potential role in the growth and survival of malignant plasma cells.