中文摘要：

目的研究增殖诱导配体（aproliferation．inducingligand，APRIL）对结直肠癌（colorec—talcancer，CRC）细胞转移侵袭能力和基质金属蛋白酶（matrixmetalloproteinases，MMPs）表达的影响，以进一步明确APRIL在CRC转移中的作用。方法APRIL基因的小干扰RNA质粒载体（siRNA—APRIL）转染人CRC细胞SW480，APRIL重组蛋白（rhAPRIL）刺激CRC细胞HCT-116，Transwell小室转移及侵袭试验分析APRIL对CRC细胞转移及侵袭能力的影响；RT-PCR、ELISA检测MMPs的表达变化。结果siRNA．APRIL转染的SW480细胞Transwell小室试验转移及侵袭细胞数显著减少（P〈0．05），rhAPRIL刺激的HCT-116细胞TransweH小室试验转移及侵袭细胞数显著增多（P〈0．05）；MMP-2、MMP-9及TIMP-1mRNA，分泌型的MMP-2、MMP-9蛋白表达在转染或刺激前后差别均有统计学意义（P〈O．05）；MMP的抑制剂GM6001处理后，SW480对照组和rhAPRIL刺激后的HCT-116细胞Transwell小室侵袭细胞数显著减少（P〈O．05）。结论APRIL通过调节MMPs的表达促进结直肠癌的侵袭和转移，可为结直肠癌转移的干预及治疗提供新的靶点。

英文摘要：

Objective To investigate the effects of a proliferation-inducing ligand（APRIL） on mi- gration and invasion of colorectal cancer（CRC） and matrix metalloproteinases（MMPs） expression in order to observe the role of APRIL in CRC metastasis. Methods The siRNA plasmid vector targeting APRIL gene （siRNA-APRIL） was transfected into SW480 cells and recombinant human APRIL（rbAPRIL） was used to stimulate HCT-116 cells. Tumor cell migration and invasion were measured by Transwell chambers. RT-PCR and ELISA were applied to examine the expression level of MMPs. Results Metastatic and invasive capaci- ties of siRNA-APRIL transfected SW480 were significantly inhibited, and these capacities of APRIL stimula- ted HCT-116 cells were siguificantly enhanced compared with their respective controls（ all P〈0.05 ）, accom- panied with the alterations of MMPs mRNA and secreted protein expression（ P〈0.05 ）. The number of inva- ding cells of SW480 control and rhAPRIL stimulated HCT-116 was significantly decreased by a MMP inhibi- tor GM6001 （P〈0. 05）. Conclusion APRIL facilitates migration and invasion of CRC via regulation of MMPs, which suggests that APRIL might be used as a new target for the intervention and treatment of CRC metastasis.